BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

BBa_J13076
BBa_J13076 Version 1 (Component)
Monocistronic CFP/YFP expression cassette

BBa_K2005051
BBa_K2005051 Version 1 (Component)
mCherry with T7 expression (oxidation-resistant)

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

BBa_K1323019
BBa_K1323019 Version 1 (Component)
Hfq expression cassette under a xylose inducible promoter

BBa_K1438010
BBa_K1438010 Version 1 (Component)
Bacterial Iron Storage Expressor

BBa_K812132
BBa_K812132 Version 1 (Component)
mCFP with kozak sequence for expression in Xenopus

BBa_K812133
BBa_K812133 Version 1 (Component)
sfGFP with kozak sequence for expression in Xenopus

BBa_K1363200
BBa_K1363200 Version 1 (Component)
Anti-LPS factor(LALF) regulated by lacI

BBa_K812130
BBa_K812130 Version 1 (Component)
Citrine reporter with a Kozak sequence for expression in Xenopus

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_K1650000
BBa_K1650000 Version 1 (Component)
Constitutive promoter expressing GFP (ILS 2015)

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.