BBa_K1036003
BBa_K1036003 Version 1 (Component)
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K323075
BBa_K323075 Version 1 (Component)
ATG cYFP link HIVC

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_J100173
BBa_J100173 Version 1 (Component)
Designed to optimize testing of fitness genes

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

BBa_K936012
BBa_K936012 Version 1 (Component)
Leader sequence that brings protein to periplasm

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_K1106013
BBa_K1106013 Version 1 (Component)
pag activator from PSP3 phage generator

BBa_K2172009
BBa_K2172009 Version 1 (Component)
Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K1657006
BBa_K1657006 Version 1 (Component)
It is called GAB. It have the resistance to glyphosate and glufosinate

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.