BBa_J72186BBa_J72186 Version 1 (Component)TrimR, pir expression cassette for genomic integration
BBa_J72188BBa_J72188 Version 1 (Component)weak violacein expression cassette
BBa_J72189BBa_J72189 Version 1 (Component)strong violacein expression cassette
BBa_J72190BBa_J72190 Version 1 (Component)neuS expression cassette
BBa_J72191BBa_J72191 Version 1 (Component)wbbL expression cassette
BBa_J72192BBa_J72192 Version 1 (Component)manB and neuS expression cassette
BBa_J72193BBa_J72193 Version 1 (Component)manB expression cassette
BBa_J72194BBa_J72194 Version 1 (Component)neuS expression cassette
BBa_J72195BBa_J72195 Version 1 (Component)Barnase/Barstar expression cassette
BBa_J72199BBa_J72199 Version 1 (Component)wbbL neuS expression cassette
BBa_J72201BBa_J72201 Version 1 (Component)BamHI endonuclease expression cassette
BBa_K812014BBa_K812014 Version 1 (Component)Auxin production device for expression in tadpole
BBa_K750000BBa_K750000 Version 1 (Component)pBADGLT:unstable gfp expression device activated by arabinose
BBa_K750001BBa_K750001 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)
BBa_K750002BBa_K750002 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)
BBa_K750003BBa_K750003 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)
BBa_K750004BBa_K750004 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)
BBa_K737038BBa_K737038 Version 1 (Component)galK::GFP generator ligated to sRNA device controlled by IPTG
BBa_K737039BBa_K737039 Version 1 (Component)galK::GFP generator ligated to sRNA device controlled by aTc
BBa_K737040BBa_K737040 Version 1 (Component)galK::GFP generator ligated to TetR generator and constitutive sRNA device
BBa_K737041BBa_K737041 Version 1 (Component)galK::GFP generator ligated to LacI generator and constitutive sRNA device
BBa_K750005BBa_K750005 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)
BBa_K750006BBa_K750006 Version 1 (Component)LuxR expression device activated by arabinose
BBa_K737058BBa_K737058 Version 1 (Component)Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose
BBa_K750008BBa_K750008 Version 1 (Component)Quorum sensing system based on LuxI and LuxR to control the expression of parts behind
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K098992BBa_K098992 Version 1 (Component)mtrB, high expression
BBa_K098990BBa_K098990 Version 1 (Component)mtrB, low expression
BBa_K098984BBa_K098984 Version 1 (Component)lac inducible mtrB (high expression of QPI)
BBa_K098983BBa_K098983 Version 1 (Component)lac inducible mtrB (low expression of QPI)
BBa_K098010BBa_K098010 Version 1 (Component)HO-pcyA, low expression
BBa_K143077BBa_K143077 Version 1 (Component)P43-gsiB GFP expression construct
BBa_K143078BBa_K143078 Version 1 (Component)P43-spoVG GFP expression construct
BBa_K143079BBa_K143079 Version 1 (Component)Pveg-spoVG GFP expression construct
BBa_K143080BBa_K143080 Version 1 (Component)P43-gsiB RFP expression construct
BBa_K143081BBa_K143081 Version 1 (Component)P43-spoVG RFP expression construct
BBa_K143082BBa_K143082 Version 1 (Component)Pveg-spoVG RFP expression construct
pBSEP1BBa_K090401 Version 1 (Component)Gram-positive Shuttle Vector for Episomal Expression
BBa_K156030BBa_K156030 Version 1 (Component)Genetic attenuator measurement device (template)
BBa_K142047BBa_K142047 Version 1 (Component)tet-controlled LacI generator with constitutive TetR expression cassette
BBa_K142024BBa_K142024 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette
BBa_K142025BBa_K142025 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette
BBa_K142026BBa_K142026 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette
BBa_K142027BBa_K142027 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette
BBa_K142028BBa_K142028 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette
BBa_K142029BBa_K142029 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette
BBa_K142030BBa_K142030 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette
BBa_K142031BBa_K142031 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette
BBa_J04430BBa_J04430 Version 1 (Component)GFP coding device switched on by IPTG
pLac+GFPBBa_I763004 Version 1 (Component)GFP coding device switched on by IPTG