BBa_K1362444
BBa_K1362444 Version 1 (Component)
GGG linker with RGK (extein)

BBa_K1916011
BBa_K1916011 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916022
BBa_K1916022 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916005
BBa_K1916005 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916008
BBa_K1916008 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916010
BBa_K1916010 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916023
BBa_K1916023 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916017
BBa_K1916017 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916015
BBa_K1916015 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916020
BBa_K1916020 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916019
BBa_K1916019 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916009
BBa_K1916009 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916001
BBa_K1916001 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916018
BBa_K1916018 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916013
BBa_K1916013 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1916007
BBa_K1916007 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1916014
BBa_K1916014 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

GFP-Si4Tag
BBa_K1602024 Version 1 (Component)
HisTag-GFP_Si4-Tag fusion protein

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

BBa_K1831001
BBa_K1831001 Version 1 (Component)
TypeIII and AFP E-coil fusion + His tag

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_I733001
BBa_I733001 Version 1 (Component)
big toe

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

BBa_K1189014
BBa_K1189014 Version 1 (Component)
GFP fused to an E coil under a lacI promoter

BBa_P10305
BBa_P10305 Version 1 (Component)
GFP

BBa_P10303
BBa_P10303 Version 1 (Component)
GFP

BBa_K1462510
BBa_K1462510 Version 1 (Component)
GAL1+YFP+SH3-lig+ADH1

BBa_K1051208
BBa_K1051208 Version 1 (Component)
The degradation tag in E. coil ,M0052 with TAATAA.

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

BBa_J52021
BBa_J52021 Version 1 (Component)
dnTraf6-linker-GFP

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_I715039
BBa_I715039 Version 1 (Component)
pLac-RBS-RFP

HisTag-GFP
BBa_K1602021 Version 1 (Component)
Green fluorescent protein (GFP) with N-terminal His-Tag

BBa_I766210
BBa_I766210 Version 1 (Component)
GFP

BBa_K176107
BBa_K176107 Version 1 (Component)
CI Inverter + GFP Reporter: PoPS->cI-LVA+pCI->GFP

BBa_T9002
BBa_T9002 Version 1 (Component)
GFP Producer Controlled by 3OC6HSL Receiver Device