BBa_I739010
BBa_I739010 Version 1 (Component)
Double regulated expression cassette for RFP +LVA (I739106.B0034.J04051.B0015)

BBa_M50050
BBa_M50050 Version 1 (Component)
IPTG-Inducible LuxS Expression in E. coli for Controlling Growth Rates

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K091212
BBa_K091212 Version 1 (Component)
lacI promoter under the control of wild-type Lac repressor

BBa_K2082006
BBa_K2082006 Version 1 (Component)
Nanobody library: Fusion proteins of Nanobody and omega subunit of RNA polymerase (rpoZ)

BBa_K1551000
BBa_K1551000 Version 1 (Component)
To generate delta-4 fatty acid Desaturase so as to synthesize the DHA.

BBa_K2082005
BBa_K2082005 Version 1 (Component)
Monobody library: Fusion proteins of Monobody and omega subunit of RNA polymerase (rpoZ)

BBa_I737007
BBa_I737007 Version 1 (Component)
Constitutive expression of all the accessory parts needed for our plasmid

BBa_K177035
BBa_K177035 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)

BBa_K750110
BBa_K750110 Version 1 (Component)
GFP(lva) expression system controlled by cI and atc(RBS strength:0.3)

BBa_K750112
BBa_K750112 Version 1 (Component)
GFP(lva) expression system controlled by cI or atc(RBS strength:1.0)

BBa_K874103
BBa_K874103 Version 1 (Component)
Arabinose inducible expression of PZF3838, M.ScaI Fusion protein (ARA -> PZF3838, M.ScaI FP)

BBa_K2117005
BBa_K2117005 Version 1 (Component)
Device encoding TEF1 and hrGFP for expression in <i>Yarrowia lipolytica</i>

BBa_K1963002
BBa_K1963002 Version 1 (Component)
A template for expression of sRNA fusions to the E. coli micC hairpin

BBa_K1507013
BBa_K1507013 Version 1 (Component)
Fatty acid induced expression auto regulator (J23119-B0034-FadR-B0015- J23119-B0034-FadL-B0015)

BBa_K1039023
BBa_K1039023 Version 1 (Component)
BXB1 integrase under lock and key control with lacI expression cassette

BBa_K1039024
BBa_K1039024 Version 1 (Component)
PhiC31 integrase under lock and key control with lacI expression cassette

BBa_K511910
BBa_K511910 Version 1 (Component)
Inducible LacI-KRAB Repressor Generator (TRE-Tight-LacI-KRAB) MammoBlock Device

BBa_K1725100
BBa_K1725100 Version 1 (Component)
SrpR repressible promoter + weak RBS + PhlF repressor + terminator + PhlF repressible promoter + wea

BBa_K1725101
BBa_K1725101 Version 1 (Component)
PhlF repressible promoter + weak RBS + SrpR repressor + terminator + SrpR repressible promoter + wea

BBa_K1963009
BBa_K1963009 Version 1 (Component)
Device for rhamnose-dependent expression of an OsmY fusion to Serratia Hfq.

BBa_K750106
BBa_K750106 Version 1 (Component)
pBADcIT-0.3:cI protein expression system activated by arabinose(RBS strength:0.3)

BBa_K750105
BBa_K750105 Version 1 (Component)
pBADcIT-0.6:cI protein expression system activated by arabinose(RBS strength:0.6)

BBa_K1962012
BBa_K1962012 Version 1 (Component)
A device for expression of Im-E3 in response to bile salts

BBa_K1962011
BBa_K1962011 Version 1 (Component)
A device for expression of Im-Ia in response to bile salts

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

BBa_K648130
BBa_K648130 Version 1 (Component)
Pr of Or tester with cI repressor with very weak RBS

BBa_K648129
BBa_K648129 Version 1 (Component)
Pr of Or tester with cI repressor with slightly weak RBS

BBa_K1431101
BBa_K1431101 Version 1 (Component)
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

BBa_K1725346
BBa_K1725346 Version 1 (Component)
<i>luxC</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725349
BBa_K1725349 Version 1 (Component)
<i>luxE</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725347
BBa_K1725347 Version 1 (Component)
<i>luxG</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725348
BBa_K1725348 Version 1 (Component)
<i>luxD</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K1725345
BBa_K1725345 Version 1 (Component)
<i>luxB</i> with optimised RBS for expression in <i>E. coli</i>

BBa_K539673
BBa_K539673 Version 1 (Component)
a RBS (B0032)+ilvC+a RBS (B0032)+ilvD with RNA thermometer+tetR+terminator

BBa_K539674
BBa_K539674 Version 1 (Component)
a RBS (B0030)+ilvC+a RBS (B0030)+ilvD with RNA thermometer+tetR+terminator

ArsR Gen.
BBa_K935001 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene and double terminator (B0010-B0012)

BBa_K1963008
BBa_K1963008 Version 1 (Component)
Device for rhamnose-dependent expression of an OsmY fusion to E. coli Hfq..

BBa_K1758313
BBa_K1758313 Version 1 (Component)
Chromium repressor under control of constitutive promoter and strong RBS,chromium responsive promote

BBa_K242300
BBa_K242300 Version 1 (Component)
multi-Ler binding site +promoter 1

BBa_K1932007
BBa_K1932007 Version 1 (Component)
This device is constructed for the expression of TAT-apoptin fused with tmp1.

BBa_M36916
BBa_M36916 Version 1 (Component)
This series of parts codes for the expression of the benzylalcohol acetyltransferase enzyme

PcotYZ
BBa_K823030 Version 1 (Component)
P_<i>cotYZ</i>: <i>B. subtilis</i> promoter regulating expression of spore crust proteins

BBa_K187254
BBa_K187254 Version 1 (Component)
folD, ORF, forward primer

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1507015
BBa_K1507015 Version 1 (Component)
Fatty acid induced expression auto regulator (pfadBA-B0034-C0051-R0051-B0034-E0040-B0015)

BBa_K1753000
BBa_K1753000 Version 1 (Component)
the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette

BBa_K1507014
BBa_K1507014 Version 1 (Component)
Fatty acid induced expression auto regulator (pfadBA-B0030-C0051-R0051-B0034-E0040-B0015)

BBa_K2055014
BBa_K2055014 Version 1 (Component)
Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron.