BBa_M11511
BBa_M11511 Version 1 (Component)
Copper Promoter (CueR regulated) with GFP Reporter

BBa_M11514
BBa_M11514 Version 1 (Component)
Copper Promoter (CueR regulated) with RFP Reporter

BBa_M11513
BBa_M11513 Version 1 (Component)
Copper Promoter (CueR regulated) with CFP Reporter

BBa_M11512
BBa_M11512 Version 1 (Component)
Copper Promoter (CueR regulated) with YFP Reporter

BBa_J15104
BBa_J15104 Version 1 (Component)
cueR copper-dependent activator coding sequence from E. coli

BBa_K1467300
BBa_K1467300 Version 1 (Component)
Golden Gate Module Flipper for 3'UTR+Terminator parts

BBa_K1811777
BBa_K1811777 Version 1 (Component)
CUP1 copper resistance gene with RBS and promoter

BBa_K1688015
BBa_K1688015 Version 1 (Component)
Nah7 - Upper naphthalene breakdown pathway (inc RBS)

BBa_J176061
BBa_J176061 Version 1 (Component)
luc miR sensor 9 (R/CMV-T/CMV)

BBa_J176058
BBa_J176058 Version 1 (Component)
luc miR sensor 6 (R/HPK-T/CMV)

BBa_J176056
BBa_J176056 Version 1 (Component)
luc miR sensor 4 (R/HPK-T/Ubc)

BBa_J176053
BBa_J176053 Version 1 (Component)
luc miR sensor 1 (R/Ubc-T/Ubc)

BBa_J176057
BBa_J176057 Version 1 (Component)
luc miR sensor 5 (R/HPK-T/HPK)

BBa_J176059
BBa_J176059 Version 1 (Component)
luc miR sensor 7 (R/CMV-T/Ubc)

BBa_J176054
BBa_J176054 Version 1 (Component)
luc miR sensor 2 (R/Ubc-T/HPK)

BBa_J176055
BBa_J176055 Version 1 (Component)
luc miR sensor 3 (R/Ubc-T/CMV)

BBa_K823050
BBa_K823050 Version 1 (Component)
luc luciferase, codon optimized for Bacillus subtilis+ RBS+Term

BBa_K119004
BBa_K119004 Version 1 (Component)
Upper primer for aiiA with normal lacZ promoter

Primer aii
BBa_K119005 Version 1 (Component)
Upper primer for aiiA with mutated lacZ promoter

BBa_K1688017
BBa_K1688017 Version 1 (Component)
Nah7 - Mutagenized upper naphthalene breakdown pathway (inc RBS)

BBa_K1811888
BBa_K1811888 Version 1 (Component)
codon optimized CUP1 copper resistance gene with RBS and promoter

BBa_K1688014
BBa_K1688014 Version 1 (Component)
Nah7 - Upper naphthalene breakdown pathway (inc RBS, J23101 promoter)

BBa_K1688013
BBa_K1688013 Version 1 (Component)
Nah7 - Upper naphthalene breakdown pathway (inc RBS, J23110 promoter)

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.