c-di-AMP
BBa_K1045002 Version 1 (Component)
CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch

BBa_K2122000
BBa_K2122000 Version 1 (Component)
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter

BBa_K2055016
BBa_K2055016 Version 1 (Component)
Part designed for verification and characterization of the FUR/LacI inverter

BBa_K1638013
BBa_K1638013 Version 1 (Component)
Leucine zipper fused to T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i>

BBa_K1638011
BBa_K1638011 Version 1 (Component)
Leucine zipper fused to T18 domain of <i>cyaA</i> from <i>Bordetella pertussis</i>

BBa_K1039020
BBa_K1039020 Version 1 (Component)
pVIII of M13 Bacteriophage with strong constitutive promoter (J23104) and RBS (B0034)

BBa_K880003
BBa_K880003 Version 1 (Component)
Asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites

BBa_K1935013
BBa_K1935013 Version 1 (Component)
White Collar Complex under control of C.elegans's promoter (pUNC119::WC1::P2A::WC2)

BBa_K346089
BBa_K346089 Version 1 (Component)
T7 promoter(BBa_I719005)+MerT+MerP+MerC(the facilitation module of mercury absorption)

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

BBa_K1314011
BBa_K1314011 Version 1 (Component)
random sequence+T7 promoter+strong rbs+ amilCP, blue chromoprotein+B0015+part of mRFP

BBa_K1779203
BBa_K1779203 Version 1 (Component)
A gene from magnetotactic bacteria which associated with the formation of the magnetosome.

BBa_K1678007
BBa_K1678007 Version 1 (Component)
Vitamin B12 biosensor, composed by a cobalamin riboswitch in front of an mRFP1

BBa_K1758313
BBa_K1758313 Version 1 (Component)
Chromium repressor under control of constitutive promoter and strong RBS,chromium responsive promote

BBa_K525562
BBa_K525562 Version 1 (Component)
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB with middle strong promoter,RBS

BBa_K1613012
BBa_K1613012 Version 1 (Component)
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.

BBa_K310006
BBa_K310006 Version 1 (Component)
ArsR under the control of J23100, includes RBS B0034 and terminator B0015

BBa_K1113401
BBa_K1113401 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

tuned pArs
BBa_K935002 Version 1 (Component)
E. coli ArsR under the control of pArs coupled with pArs + RFP

CspB | RFP
BBa_K525234 Version 1 (Component)
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS

BBa_K1431101
BBa_K1431101 Version 1 (Component)
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

BBa_K782048
BBa_K782048 Version 1 (Component)
HindIII/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K782050
BBa_K782050 Version 1 (Component)
BamHI/XbaI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782049
BBa_K782049 Version 1 (Component)
HindIII/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K782051
BBa_K782051 Version 1 (Component)
BamHI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782052
BBa_K782052 Version 1 (Component)
BamHI/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782058
BBa_K782058 Version 1 (Component)
SacI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K525264
BBa_K525264 Version 1 (Component)
Fusion Protein of Luciferase, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7

M13 -47
BBa_K176059 Version 1 (Component)
M13 -47 general primer as a reverse primer binds to 5prime terminal of lacZ

BBa_K782044
BBa_K782044 Version 1 (Component)
EcoRI/XbaI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782057
BBa_K782057 Version 1 (Component)
SacI/SpeI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_J119408
BBa_J119408 Version 1 (Component)
Pupp promoter mutant - Substitution of C and G to A at 28 and 30

BBa_K782043
BBa_K782043 Version 1 (Component)
EcoRI/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K525261
BBa_K525261 Version 1 (Component)
Fusion Protein of Luciferase and cspB from Corynebacterium halotolerans with TAT-Sequence and lipid

BBa_K782055
BBa_K782055 Version 1 (Component)
XbaI/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

mdnA-myc-g
BBa_K627006 Version 1 (Component)
Fusion part of mdnA gene (from mdn-cluster) with myc-tag and gene III

BBa_K782053
BBa_K782053 Version 1 (Component)
XbaI/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K2170140
BBa_K2170140 Version 1 (Component)
N-terminal part of prokaryotic biotin binding receptor (TetR_TetO_OmpA_eMA) in RFC[25] N-part

BBa_K782046
BBa_K782046 Version 1 (Component)
HindIII/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vecto

BBa_K782054
BBa_K782054 Version 1 (Component)
XbaI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782045
BBa_K782045 Version 1 (Component)
EcoRI/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K357007
BBa_K357007 Version 1 (Component)
Fusion of muconolactone D-isomerase (Enzyme 3) and 3-oxoadipate enol-lactonase (Enzyme 4)

BBa_K782056
BBa_K782056 Version 1 (Component)
SacI/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782047
BBa_K782047 Version 1 (Component)
HindIII/XbaI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1935016
BBa_K1935016 Version 1 (Component)
White Collar 2 under the control of pU6 promoter in C.elegans (pU6::WC2)

BBa_K1465209
BBa_K1465209 Version 1 (Component)
Carbonic anhydrase (csoS3), shell-proteins (csoS14) and pore protein (csoS1D) of the carboxysome

BBa_K1935014
BBa_K1935014 Version 1 (Component)
White Collar 1 under the control of pU6 promoter in C.elegans (pU6::WC1)

BBa_K182003
BBa_K182003 Version 1 (Component)
Intermediate part consisting of CI promoter followed by tetR, LacI and Tet Operator

BBa_K2082232
BBa_K2082232 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with Gal11P:cI(434)