BBa_K624049
BBa_K624049 Version 1 (Component)
RBS(Pmsp3) + minC + RBS(Pmsp3) +mcherry

BBa_K1650011
BBa_K1650011 Version 1 (Component)
sRNA Anti-MinC leading to Minicell Production

BBa_K1937008
BBa_K1937008 Version 1 (Component)
AF-A antifungal operon in pSBBs0K-mini

BBa_K2129002
BBa_K2129002 Version 1 (Component)
Mutant EutS with Mid expression cassette

BBa_K1480006
BBa_K1480006 Version 1 (Component)
pSB1C3 + MID TALEN (K1480001) Target site

BBa_K1480013
BBa_K1480013 Version 1 (Component)
pSB3C5 + MID TALEN (K1480001) Target site

BBa_K1937010
BBa_K1937010 Version 1 (Component)
Epsilon/MazF toxin and antitoxin in pSBBS0K-mini

BBa_K1093024
BBa_K1093024 Version 1 (Component)
MinC/Lysis device-based kill switch with delayed expression

BBa_K624052
BBa_K624052 Version 1 (Component)
Pmsp1(tetO) + RBS(trunc.) + minC + RBS(trunc.) + mcherry

BBa_K624045
BBa_K624045 Version 1 (Component)
Pmsp1(tetO) + RBS(Pmsp3) + minC + RBS(Pmsp3) + mcherry

BBa_K624053
BBa_K624053 Version 1 (Component)
mcherry+minC+RBS+tetO+pT7 + pT7+RBS+tetR+GFP

BBa_K624054
BBa_K624054 Version 1 (Component)
mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP

BBa_K360141
BBa_K360141 Version 1 (Component)
Min. Blue Promoter + RBS + GFP BBa_E0040

VR8
BBa_K176052 Version 1 (Component)
Reverse primer VR8 (bind to K176042), member of VR3~VR12

VR3
BBa_K176047 Version 1 (Component)
Reverse primer VR3 (bind to K176037), member of VR3~VR12

VR10
BBa_K176054 Version 1 (Component)
Reverse primer VR10 (bind to K176044), member of VR3~VR12

VR4
BBa_K176048 Version 1 (Component)
Reverse primer VR4 (bind to K176038), member of VR3~VR12

VR11
BBa_K176055 Version 1 (Component)
Reverse primer VR11 (bind to K176045), member of VR3~VR12

VR12
BBa_K176056 Version 1 (Component)
Reverse primer VR12 (bind to K176046), member of VR3~VR12

VR5
BBa_K176049 Version 1 (Component)
Reverse primer VR5 (bind to K176039), member of VR3~VR12

VR7
BBa_K176051 Version 1 (Component)
Reverse primer VR7 (bind to K176041), member of VR3~VR12

VR9
BBa_K176053 Version 1 (Component)
Reverse primer VR9 (bind to K176043), member of VR3~VR12

VR6
BBa_K176050 Version 1 (Component)
Reverse primer VR6 (bind to K176040), member of VR3~VR12

BBa_K1485002
BBa_K1485002 Version 1 (Component)
A kind of GFP for eukaryotes with a linker (GS)x5

BBa_K1480019
BBa_K1480019 Version 1 (Component)
pSB3C5 + MID + BF + AF TALEN (K1480001) Target sites

BBa_K1480017
BBa_K1480017 Version 1 (Component)
pSB3C5 + MID and AF TALEN (K1480001) Target sites

BBa_K1480016
BBa_K1480016 Version 1 (Component)
pSB3C5 + Before and MID TALEN (K1480001) Target site

BBa_K1480007
BBa_K1480007 Version 1 (Component)
pSB1C3 + MID and AF TALEN (K1480001) Target sites

BBa_K1480009
BBa_K1480009 Version 1 (Component)
pSB1C3 + MID and BF TALEN (K1480001) Target sites

BBa_K360044
BBa_K360044 Version 1 (Component)
Min. Blue Promoter BBa_K360041 + Mutated Red Luciferase BBa_K360115

BBa_K299806
BBa_K299806 Version 1 (Component)
minC cell division inhibitor

BBa_K1480008
BBa_K1480008 Version 1 (Component)
pSB1C3 + MID, AF and BF TALEN (K1480001) Target sites

BBa_K1431503
BBa_K1431503 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431501
BBa_K1431501 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431502
BBa_K1431502 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_J29040
BBa_J29040 Version 1 (Component)
MinC

GFP-ASV
BBa_K1639023 Version 1 (Component)
GFP with an ASV degradation tag with a half-life of ~110 min

BBa_K2088006
BBa_K2088006 Version 1 (Component)
It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I

BBa_K1088009
BBa_K1088009 Version 1 (Component)
B. subtilis dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG ind

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.