BBa_K1088002
BBa_K1088002 Version 1 (Component)
Reporter system to quantify protein expression of B. sub 1-deoxyxylulose-5-phosphate synthase (Dxs)

PhyB-908-(
BBa_K365011 Version 1 (Component)
Phytochrome B (aa 1-908) linked to a trimer of ClpX (aa 61-425)

PB642(L-C)
BBa_K365010 Version 1 (Component)
Phytochrome B (aa 1-642) linked to a trimer of ClpX (aa 61-425)

BBa_K1453304
BBa_K1453304 Version 1 (Component)
pBluescript II KS(+)_5_copy

BBa_K1453303
BBa_K1453303 Version 1 (Component)
pBluescript II KS(+)_3_copy

BBa_K1676120
BBa_K1676120 Version 1 (Component)
Mutant 16 of Lactate Dehydrogenase

BBa_K1676114
BBa_K1676114 Version 1 (Component)
Mutant 10 of Lactate Dehydrogenase

PcotV
BBa_K823033 Version 1 (Component)
P_<i>cotV</i>: <i>B. subtilis</i> promoter regulates spore crust protein cotV

BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter

Strep-Tag
BBa_I757014 Version 1 (Component)
Strep-Tag II affinity tag

Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K1088009
BBa_K1088009 Version 1 (Component)
B. subtilis dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG ind

BBa_S04314
BBa_S04314 Version 1 (Component)
RBS + Blh Gene

CAD1en2
BBa_K1658001 Version 1 (Component)
Cinnamyl alcohol dehydrogenase CAD1 - 2

BBa_M31114
BBa_M31114 Version 1 (Component)
M13, gene II from HpaI to the end [1-831bp]

BBa_K549004
BBa_K549004 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_K1676088
BBa_K1676088 Version 1 (Component)
Mutant 12 of LDH and wildtype LDP

BBa_K1676091
BBa_K1676091 Version 1 (Component)
Mutant 15 of LDH and wildtype LDP

BBa_K1676086
BBa_K1676086 Version 1 (Component)
Mutant 10 of LDH and wildtype LDP

BBa_K1676080
BBa_K1676080 Version 1 (Component)
Mutant 4 of LDH and wildtype LDP

BBa_K1444011
BBa_K1444011 Version 1 (Component)
Composite promoter and consensus B. subtilis RBS - pTetR

BBa_K1444014
BBa_K1444014 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - pTetR x2

BBa_K1444010
BBa_K1444010 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - C1-434

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.