BBa_K1165004
BBa_K1165004 Version 1 (Component)
PenI-cleaving Ribozyme 1 of XOR Gate 2

BBa_K1165002
BBa_K1165002 Version 1 (Component)
PenI-cleaving Ribozyme 1 of XOR Gate 1

BBa_K1392972
BBa_K1392972 Version 1 (Component)
Composite part fot TetLVA TetPromoter and T4

BBa_K648034
BBa_K648034 Version 1 (Component)
Or tester

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1109007
BBa_K1109007 Version 1 (Component)
FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence transc

BBa_K258012
BBa_K258012 Version 1 (Component)
AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism

BBa_K525552
BBa_K525552 Version 1 (Component)
Polycistronic expression of FNR, BisdA and BisdB

BBa_K195413
BBa_K195413 Version 1 (Component)
All in one

BBa_K1163400
BBa_K1163400 Version 1 (Component)
AceB promoter, iron sensitive, down-regulated by dimerized FUR protein

BBa_I732999
BBa_I732999 Version 1 (Component)
Test for script

BBa_J100099
BBa_J100099 Version 1 (Component)
A promoter (CydAB) activated by the FNR enzyme

BBa_K239002
BBa_K239002 Version 1 (Component)
HypB promoter, activated by Fnr during oxygen deficiency

BBa_K1970001
BBa_K1970001 Version 1 (Component)
XOR gate like repressor of CI repressor expression

BBa_K1132032
BBa_K1132032 Version 1 (Component)
Test of the XOR gate (BBa_K1132002) with RFP

BBa_K257024
BBa_K257024 Version 1 (Component)
Jun : first part of the dimere Jun/Fos

BBa_K1132002
BBa_K1132002 Version 1 (Component)
XOR gate with recombinases switching gene regulatory sequences

BBa_K415006
BBa_K415006 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term

BBa_K1479006
BBa_K1479006 Version 1 (Component)
Just for upload test

cre
BBa_K112102 Version 1 (Component)
Cre enzyme without start or stop codons

BBa_K188006
BBa_K188006 Version 1 (Component)
Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be

luxR-ndh
BBa_K1036000 Version 1 (Component)
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_K1956026
BBa_K1956026 Version 1 (Component)
Noise in Device measurement

BBa_K1956025
BBa_K1956025 Version 1 (Component)
Noise in Device measurement

BBa_K1956024
BBa_K1956024 Version 1 (Component)
Noise in Device measurement

BBa_K1956027
BBa_K1956027 Version 1 (Component)
Noise in Device measurement

BBa_M1259
BBa_M1259 Version 1 (Component)
gluteomorphin or gliadorphin

BBa_K1743002
BBa_K1743002 Version 1 (Component)
Plastocyanin variant #3 in pSB1C3

BBa_K1743012
BBa_K1743012 Version 1 (Component)
Plastocyanin variant #2 in pSB1C3

BBa_K1743014
BBa_K1743014 Version 1 (Component)
Plastocyanin variant #4 in pSB1C3

BBa_K1743015
BBa_K1743015 Version 1 (Component)
Plastocyanin variant #5 in pSB1C3

BBa_K1743004
BBa_K1743004 Version 1 (Component)
Plastocyanin variant #1 in pSB1C3

BBa_K112105
BBa_K112105 Version 1 (Component)
BamHI endonuclease without start or stop codons

BBa_K2044010
BBa_K2044010 Version 1 (Component)
Based on our project, <5,7> is the direct pathway from Site No.5to Site No.7 in the map we design.

BBa_I9111
BBa_I9111 Version 1 (Component)
test (lambda-cI nor) I9110.I9109.I9109

BBa_I9108
BBa_I9108 Version 1 (Component)
test (tp901-cI nor) I9107.I9106.I9106

BBa_K415019
BBa_K415019 Version 1 (Component)
pLuxR/cI-OR : RBS : LuxI

BBa_J64974
BBa_J64974 Version 1 (Component)
small molecule controlled, riboregulator based XOR gate

BBa_K239003
BBa_K239003 Version 1 (Component)
Modified HypB promoter, activated by Fnr during oxygen deficiency

BBa_I711026
BBa_I711026 Version 1 (Component)
Amy is learning how to build a biobrick. Hor.

BBa_M1183
BBa_M1183 Version 1 (Component)
Practice for Class

BBa_K187023
BBa_K187023 Version 1 (Component)
GFP in pBA, BioBytes plasmid

BBa_K187019
BBa_K187019 Version 1 (Component)
RFP in pAB, Biobytes plasmid

BBa_K187026
BBa_K187026 Version 1 (Component)
terminator in pBA, Biobytes plasmid

BBa_K112306
BBa_K112306 Version 1 (Component)
{lambda holin!} in BBb format

BBa_K415022
BBa_K415022 Version 1 (Component)
PluxR/cI-OR : RBS : mCherry : Term : PtetR : RBS : LuxR : Term : Plux/cI-OR : RBS : LuxI