FBS-AceB+L
BBa_K1163999 Version 1 (Component)
Inverter composed of Fur Binding site from AceB promoter + LacI-LVA

BBa_I730000
BBa_I730000 Version 1 (Component)
Feruloyl CoA hydratase for vanillin biosynthesis

BBa_K1795400
BBa_K1795400 Version 1 (Component)
Truncated pSB1C3 with no Prefix or Suffix

mCherry
BBa_K105010 Version 1 (Component)
mCherry, Yeast optimized for fusion proteins

BBa_K1363608
BBa_K1363608 Version 1 (Component)
gate of or of RNA logic gates

BBa_K105008
BBa_K105008 Version 1 (Component)
EYFP, yeast optimized for fusion proteins

BBa_I738002
BBa_I738002 Version 1 (Component)
feruloyl-CoA hydratase enzyme for biosynthesis of vanillin

BBa_K648127
BBa_K648127 Version 1 (Component)
Or tester with cI repressor

BBa_J100091
BBa_J100091 Version 1 (Component)
For Testing New Promoters via Golden Gate Assembly

BBa_J70427
BBa_J70427 Version 1 (Component)
R0010.J70400.E0020 or J70406.E0020

BBa_J70426
BBa_J70426 Version 1 (Component)
R0010.J70400.E0030 or J70406.E0030

BBa_K415020
BBa_K415020 Version 1 (Component)
PluxR/cI-OR : RBS : mCherry: Term : PtetR : RBS : LuxR : Term : PLuxR/cI-OR : RBS : LuxI

BBa_M36914
BBa_M36914 Version 1 (Component)
Codes for the benzylalcohol acetyltransferase enzyme

BBa_J70434
BBa_J70434 Version 1 (Component)
R0010.J70428.E0020 or J70431.E0020

BBa_J70433
BBa_J70433 Version 1 (Component)
R0010.J70428.E0030 or J70431.E0030

BBa_J70425
BBa_J70425 Version 1 (Component)
R0010.J70399.E0020 or J70405.E0020

BBa_J70432
BBa_J70432 Version 1 (Component)
R0010.J70428.E0040 or J70431.E0040

BBa_J70424
BBa_J70424 Version 1 (Component)
R0010.J70399.E0030 or J70405.E0030

BBa_K648030
BBa_K648030 Version 1 (Component)
Or with RBS B0034 and Mcherry

BBa_J70423
BBa_J70423 Version 1 (Component)
R0010.J70399.E0040 or J70405.E0040

BBa_K1366101
BBa_K1366101 Version 1 (Component)
Genetic construct for lpp gene deletion (C1)

BBa_K619888
BBa_K619888 Version 1 (Component)
RNA Thermometer or thermosensor (30-37 Celsius)

BBa_K1363609
BBa_K1363609 Version 1 (Component)
gate of or of RNA logic gates

BBa_I754001
BBa_I754001 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_I769106
BBa_I769106 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_J24820
BBa_J24820 Version 1 (Component)
Renilla luciferase reporter device for DLARAP

BBa_K2180012
BBa_K2180012 Version 1 (Component)
Promoter PlepA +FUR regulatory sequence + RBS + LacI-LVA + Terminator + Pigment + RBS + Operateur La

BBa_K1884009
BBa_K1884009 Version 1 (Component)
PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator

BBa_J07031
BBa_J07031 Version 1 (Component)
Antirabbit IgG scFV: no Pst1 or Not1 sites

BBa_K587008
BBa_K587008 Version 1 (Component)
Crxst -> Lock sequence for low concentration mechanism

BBa_K648108
BBa_K648108 Version 1 (Component)
RecA without restriction sites or recombinant activity

BBa_K357002
BBa_K357002 Version 1 (Component)
muconolactone Delta-isomerase or muconolactone D-isomerase

BBa_K648132
BBa_K648132 Version 1 (Component)
Pr of Or tester with Cro

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J70405
BBa_J70405 Version 1 (Component)
R0010.J70399 (Ribosome Replacer or Tag Adder)

BBa_K116500
BBa_K116500 Version 1 (Component)
OmpF promoter that is activated or repressesed by OmpR according to osmolarity.

BBa_I735000
BBa_I735000 Version 1 (Component)
ech gene coding sequence for feruoyl CoA hydratese

BBa_K2184029
BBa_K2184029 Version 1 (Component)
SNP for non taster NaCl & Sour -TAS1R1 rs17492553

BBa_K292009
BBa_K292009 Version 1 (Component)
Stem cell factor (SCF) or c-kit-ligand

BBa_K1363610
BBa_K1363610 Version 1 (Component)
key-1-of-or of RNA logic gates

BBa_K415069
BBa_K415069 Version 1 (Component)
pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Fixed-pLux/cI:LuxI

cln2
BBa_K105014 Version 1 (Component)
cln2 PEST destabilization domain for rapid protein turnover

BBa_K415007
BBa_K415007 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : pLux/cI-OR : RBS-luxI : Term : p(tetR) : RBS-luxR : Term

BBa_J24818
BBa_J24818 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters

BBa_J70404
BBa_J70404 Version 1 (Component)
R0010.E0021.J70394.E0031 or J70398.E0031

BBa_J70403
BBa_J70403 Version 1 (Component)
R0010.E0031.J70394.E0021 or J70397.E0021

BBa_M50016
BBa_M50016 Version 1 (Component)
Promoter, RBS and GolS Activator for Gold Detection

BBa_K648131
BBa_K648131 Version 1 (Component)
Pr of Or tester with cI repressor