Prom/RBSBBa_K262000 Version 1 (Component)BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.
BBa_K608001BBa_K608001 Version 1 (Component)First of two requisite genes required for the biosynthesis of phycocyanobilin from heme combined wit
BBa_K1088058BBa_K1088058 Version 1 (Component)10 aa linker with BamHI restriction site and TEV recognition site
BBa_K1638006BBa_K1638006 Version 1 (Component)10 aa linker with BamHI restriction site and TEV recognition site
BBa_K2123113BBa_K2123113 Version 1 (Component)Stationary phase promoter with downstream mer operator + RFP (K081014)
BBa_K779121BBa_K779121 Version 1 (Component)Short DNA reporter top strand (with RQ quencher) MammoBlock
BBa_K779116BBa_K779116 Version 1 (Component)Short RNA reporter bottom strand (with ROX fluorophore) MammoBlock
BBa_K648123BBa_K648123 Version 1 (Component)RecA with mutation of Lys 286 and Arg 243
BBa_K101012BBa_K101012 Version 1 (Component)Construct with TetR/p22cII promoter, RBS, RFP, Terminator
BBa_K549004BBa_K549004 Version 1 (Component)LacI promotor fused with the iron dependent regulator fur
BBa_K1952012BBa_K1952012 Version 1 (Component)Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter
ChiP-lacZBBa_K564005 Version 1 (Component)Chitoporin fused with lacZ -target for sRNA based regulation
PchiP-lacZBBa_K564002 Version 1 (Component)Chitoporin fused with lacZ - target for sRNA based regulation
BBa_K1033204BBa_K1033204 Version 1 (Component)pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance
BBa_J58014BBa_J58014 Version 1 (Component)Promoter activated by OmpR-P with the reporter GFP
BBa_K861012BBa_K861012 Version 1 (Component)FadD and FadL with a RBS and a terminator
BBa_K290001BBa_K290001 Version 1 (Component)constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl
BBa_K812130BBa_K812130 Version 1 (Component)Citrine reporter with a Kozak sequence for expression in Xenopus
BBa_M1729BBa_M1729 Version 1 (Component)GFP reporter expression after induction with pBAD and hepcidin.
BBa_K1989037BBa_K1989037 Version 1 (Component)Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PhoA
BBa_K300079BBa_K300079 Version 1 (Component)Phasin (PhaP) - head domain - with flexible protein domain linker downstream
BBa_J58015BBa_J58015 Version 1 (Component)Mutated promoter activated by OmpR-P with the reporter GFP
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
BBa_K2123112BBa_K2123112 Version 1 (Component)Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
GFP-AIDBBa_K812110 Version 1 (Component)GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid
BBa_K1067000BBa_K1067000 Version 1 (Component)Periplasmic directed GFP SF with signal peptide TAT and RFP as background color
BBa_J24822BBa_J24822 Version 1 (Component)Same as J24819 but with the error at the luc-terminator junction fixed
BBa_K1796201BBa_K1796201 Version 1 (Component)An unloaded sgRNA that contains BbsI cutting site, with a promoter and terminator.
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
BBa_M33334BBa_M33334 Version 1 (Component)CDS+RBS for Inorganic mercury-sensitive regulatory protein merR coding sequence with RBS
BBa_K2123115BBa_K2123115 Version 1 (Component)Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014
BBa_K779120BBa_K779120 Version 1 (Component)RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock
BBa_K323164BBa_K323164 Version 1 (Component)VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5
BBa_K2123114BBa_K2123114 Version 1 (Component)Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
GG100BBa_K2145125 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
GG98BBa_K2145123 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
BBa_K2144011BBa_K2144011 Version 1 (Component)Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter
BBa_K1088052BBa_K1088052 Version 1 (Component)GFP reporter with flexible linker at N-terminus for creation of GFP fusions
BBa_K323163BBa_K323163 Version 1 (Component)VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5
BBa_J24823BBa_J24823 Version 1 (Component)same as J24819 but with ACCACC Euk RBS removed and problem solved via J24822 removed
BBa_K1753000BBa_K1753000 Version 1 (Component)the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)
BBa_K2123116BBa_K2123116 Version 1 (Component)Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K541715BBa_K541715 Version 1 (Component)Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.