Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

LasR+PAI
BBa_R0079 Version 1 (Component)
Promoter (LasR & PAI regulated)

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>

BBa_K314100
BBa_K314100 Version 1 (Component)
High constitutive expression cassette

pTEF
BBa_K165037 Version 1 (Component)
TEF2 yeast constitutive promoter

BBa_I13521
BBa_I13521 Version 1 (Component)
Ptet mRFP

lasI
BBa_C0178 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)

kanR
BBa_P1003 Version 1 (Component)
kanamycin resistance cassette

BBa_K864400
BBa_K864400 Version 1 (Component)
Ptac, trp & lac regulated promoter

BBa_S04000
BBa_S04000 Version 1 (Component)
Ptet . Las R Cassette

BBa_J61002
BBa_J61002 Version 1 (Component)
EX-Ptet-S-rbsRFP-P "RFP reporter"

BBa_J33207
BBa_J33207 Version 1 (Component)
lac promoter and lacZ

BBa_I13602
BBa_I13602 Version 1 (Component)
Tet operator with CFP reporter (with LVA tag) [R/Tc+]

BBa_K1789012
BBa_K1789012 Version 1 (Component)
P+R

BBa_I13601
BBa_I13601 Version 1 (Component)
Lac operator with CFP reporter (without LVA tag) [R/Lc-]

pLux/Tet
BBa_K176000 Version 1 (Component)
pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS

las TetO
BBa_I14015 Version 1 (Component)
P(Las) TetO

CmR
BBa_J61000 Version 1 (Component)
chloramphenicol resistance cassette

BBa_K1893000
BBa_K1893000 Version 1 (Component)
Las receiver (LasR+pLas)

BBa_K876015
BBa_K876015 Version 1 (Component)
pTet-LasR-DT

BBa_I763026
BBa_I763026 Version 1 (Component)
LacI coding device regulated by pTetR

BBa_K124017
BBa_K124017 Version 1 (Component)
Bacteriophage Lysis Cassette S105, R, and Rz

BBa_K116640
BBa_K116640 Version 1 (Component)
pTet + RBS + LuxR + T

cmR
BBa_P1004 Version 1 (Component)
chloramphenicol resistance cassette