BBa_K382068
BBa_K382068 Version 1 (Component)
intron hairpin RNAi knockdown of Bet v 1 allergen in Basta plant vector

BBa_K1363200
BBa_K1363200 Version 1 (Component)
Anti-LPS factor(LALF) regulated by lacI

BBa_K1363201
BBa_K1363201 Version 1 (Component)
Anti-LPS factor(LALF) regulated by IPTG

BBa_K541800
BBa_K541800 Version 1 (Component)
B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli

BBa_K782048
BBa_K782048 Version 1 (Component)
HindIII/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K782049
BBa_K782049 Version 1 (Component)
HindIII/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K782047
BBa_K782047 Version 1 (Component)
HindIII/XbaI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K1954007
BBa_K1954007 Version 1 (Component)
Green Light Inducible bacteriocin Device (GLID)

BBa_K1323019
BBa_K1323019 Version 1 (Component)
Hfq expression cassette under a xylose inducible promoter

BBa_K2092002
BBa_K2092002 Version 1 (Component)
PalcA, improved alcR inducible promoter from A. nidulans

BBa_K590046
BBa_K590046 Version 1 (Component)
AAR-PSB3K3-Lac Inducible w/o LacI

BBa_K1558000
BBa_K1558000 Version 1 (Component)
Clostridial shuttle vector

BBa_K638201
BBa_K638201 Version 1 (Component)
Arabinose inducible Poly-His Reflectin A1 generator

BBa_K1088010
BBa_K1088010 Version 1 (Component)
E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib

BBa_K418001
BBa_K418001 Version 1 (Component)
From partsregistry.org IPTG inducible Lac promoter cassette

Prom/RBS
BBa_K262000 Version 1 (Component)
BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.

BBa_K799024
BBa_K799024 Version 1 (Component)
pSB1C3 yGG promoter acceptor vector

BBa_K799025
BBa_K799025 Version 1 (Component)
pSB1C3 yGG ORF acceptor vector

BBa_K299509
BBa_K299509 Version 1 (Component)
Expression Vector pT7+B0034

BBa_J95039
BBa_J95039 Version 1 (Component)
plasmid vector for Rhodobacter sphaeroides

BBa_J01120
BBa_J01120 Version 1 (Component)
Sacrafical OnRFP Kan vector

BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)

BBa_K112995
BBa_K112995 Version 1 (Component)
BBb1 assembly vector - C/A

BBa_K1968009
BBa_K1968009 Version 1 (Component)
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_K1968014
BBa_K1968014 Version 1 (Component)
Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae

BBa_J72117
BBa_J72117 Version 1 (Component)
BBb High copy entry vector, pBca1256

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

BBa_K106693
BBa_K106693 Version 1 (Component)
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

BBa_K1075003
BBa_K1075003 Version 1 (Component)
Promoter(const.)-AraC-Term-pBAD-RBS34 (Arabinose inducable promoter system)

BBa_M39111
BBa_M39111 Version 1 (Component)
Vector for creation of micro-dystrophin SFV

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

BBa_K1114400
BBa_K1114400 Version 1 (Component)
This is a MoClo level 0 destination vector.

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.