BBa_K1811333
BBa_K1811333 Version 1 (Component)
CUP1 metallothionein fused to LamB on outer membrane

BBa_K137120
BBa_K137120 Version 1 (Component)
LamB and TetA expression plasmid

BBa_J100227
BBa_J100227 Version 1 (Component)
WS6 Clone C in tCloneRed

BBa_K119008
BBa_K119008 Version 1 (Component)
Lower primer for RcnA (BBa_K119003)

BBa_K129004
BBa_K129004 Version 1 (Component)
LamB_HP-metal binding domain in LamB-153 residue

BBa_K129005
BBa_K129005 Version 1 (Component)
LamB_CP-metal binding domain in LamB-153 residue

BBa_K1773026
BBa_K1773026 Version 1 (Component)
IPTG inducible cI repressor cloned with LuxI gene

BBa_I732004
BBa_I732004 Version 1 (Component)
Over-express gyrA

BBa_K1216008
BBa_K1216008 Version 1 (Component)
Variant of the wild-type pLuxR promoter with lower sensitivity

BBa_K137123
BBa_K137123 Version 1 (Component)
lamB and tetR cassette Weak Promoter+Weak RBS

BBa_K137122
BBa_K137122 Version 1 (Component)
lamB and TetR cassette Weak Promoter+Medium RBS

BBa_K137121
BBa_K137121 Version 1 (Component)
lamB and tetR cassette Medium Promoter+Weak RBS

BBa_K146001
BBa_K146001 Version 1 (Component)
AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002

BBa_K1811666
BBa_K1811666 Version 1 (Component)
CUP1 metallothionein fused to LamB with promoter and RBS

BBa_K1323020
BBa_K1323020 Version 1 (Component)
oriV from the Staphylococcus aureus pSK41 plasmid clone 3

BBa_K1532008
BBa_K1532008 Version 1 (Component)
project system we used to slove the SPP

BBa_K1216007
BBa_K1216007 Version 1 (Component)
Variant of the wild-type pLuxR promoter with lower sensitivity

BBa_K1216009
BBa_K1216009 Version 1 (Component)
Variant of the wild-type pLuxR promoter with lower sensitivity

BBa_K540000
BBa_K540000 Version 1 (Component)
<i>rcn-csgBAEFG</i>, over-induces adherence in response to cobalt

BBa_K336053
BBa_K336053 Version 1 (Component)
Over expression system (T7 polymerase induced by lac promoter)

BBa_K1961007
BBa_K1961007 Version 1 (Component)
CYP1A2, an enzyme of cytochrome P450 protein family that metabolizes toxin in liver

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.