BBa_K1487076
BBa_K1487076 Version 1 (Component)
eYFP Reporter for tcpI gRNA target with strong promoter.

BBa_K1487077
BBa_K1487077 Version 1 (Component)
eYFP Reporter for tcpJ gRNA target with strong promoter.

BBa_K1487078
BBa_K1487078 Version 1 (Component)
eYFP Reporter for tcpP gRNA target with strong promoter.

BBa_K1487079
BBa_K1487079 Version 1 (Component)
eYFP Reporter for tcpQ gRNA target with strong promoter.

BBa_K1487080
BBa_K1487080 Version 1 (Component)
eYFP Reporter for tcpR gRNA target with strong promoter.

BBa_K1487081
BBa_K1487081 Version 1 (Component)
eYFP Reporter for tcpS gRNA target with strong promoter.

BBa_K1487082
BBa_K1487082 Version 1 (Component)
eYFP Reporter for tcpT gRNA target with strong promoter.

BBa_K1487083
BBa_K1487083 Version 1 (Component)
eYFP Reporter for toxT gRNA target with strong promoter.

BBa_K1716002
BBa_K1716002 Version 1 (Component)
NahR Biosensor for detection of acetylsalicylic acid/aspirin with blue chromoprotein reporter

Coupling
Coupling Version 1 (Component)
Translational coupler

BBa_K1943004
BBa_K1943004 Version 1 (Component)
eforRed, Red chromoprotein reporter system (Strong Promoter, Strong RBS)

BBa_K1943005
BBa_K1943005 Version 1 (Component)
eforRed, Red chromoprotein reporter system (medium Promoter , weak RBS)

BBa_K1960054
BBa_K1960054 Version 1 (Component)
GFP reporter and Self-induced synthetase for AHL

BBa_K1963005
BBa_K1963005 Version 1 (Component)
A translational fusion between E. coli OsmY and E. coli Hfq

BBa_K1963006
BBa_K1963006 Version 1 (Component)
A translational fusion between E. coli OsmY and Serratia Hfq

GG95
BBa_K2145000 Version 1 (Component)
GFP/RFP reporter (Forward/Forward) with random 500bp spacer

GG96
BBa_K2145001 Version 1 (Component)
GFP/RFP reporter (Reverse/Forward) with random 500bp spacer

BBa_K2020040
BBa_K2020040 Version 1 (Component)
Fluorescent reporter for measurement of incorporation of ncAA

BBa_K2116024
BBa_K2116024 Version 1 (Component)
Dual fluorescence reporter for bxb1 integrase

BBa_K2176003
BBa_K2176003 Version 1 (Component)
yeGFP Reporter for LexA

BBa_K2182003
BBa_K2182003 Version 1 (Component)
Translational Unit for His-tagged NOX

GG105
BBa_K2145100 Version 1 (Component)
GFP/RFP reporter (Forward/Forward) separated by three dCas clamp sites

GG106
BBa_K2145101 Version 1 (Component)
GFP/RFP reporter (Reverse/Forward) separated by three dCas clamp sites

GG107
BBa_K2145102 Version 1 (Component)
GFP/RFP reporter (Forward/Reverse) separated by three dCas clamp sites

GG97
BBa_K2145104 Version 1 (Component)
GFP/RFP reporter (Forward/Reverse) with random 500bp spacer

GG100
BBa_K2145107 Version 1 (Component)
GFP/RFP reporter (Reverse/Forward) with random 500bp spacer

GG99
BBa_K2145124 Version 1 (Component)
GFP/RFP reporter (Forward/Reverse) with random 500bp spacer

GG101
BBa_K2145126 Version 1 (Component)
GFP/RFP reporter (Forward/Reverse) with random 500bp spacer

BBa_J70661
BBa_J70661 Version 1 (Component)
Reporter for assembly comparison project J70607-E0040

BBa_J133421
BBa_J133421 Version 1 (Component)
Translational Stop Sequence 1:001

BBa_J133422
BBa_J133422 Version 1 (Component)
Translational Stop Sequence 2:010

BBa_J133423
BBa_J133423 Version 1 (Component)
Translational Stop Sequence 3:011

BBa_J133424
BBa_J133424 Version 1 (Component)
Translational Stop Sequence 4:100

BBa_J133425
BBa_J133425 Version 1 (Component)
Translational Stop Sequence 5:101

BBa_J133426
BBa_J133426 Version 1 (Component)
Translational Stop Sequence 6:110

BBa_J133427
BBa_J133427 Version 1 (Component)
Translational Stop Sequence 7:111

BBa_K606014
BBa_K606014 Version 1 (Component)
RBS SpoVG GFPmut3b (reporter system for B. Subtilis)

BBa_K606013
BBa_K606013 Version 1 (Component)
SpoVG RFPmCherry double terminator. Translational unit for B. Subtilis

BBa_J85002
BBa_J85002 Version 1 (Component)
theoretical RBS with no translation of RFP

BBa_K608151
BBa_K608151 Version 1 (Component)
translational unit of pcyA

BBa_K631036
BBa_K631036 Version 1 (Component)
eCFP:UNC-54 (Reporter; for use in C. elegans)

PchiP-lacZ
BBa_K564012 Version 1 (Component)
promoter and RBS of E. coli chitoporin translationally fused with lacZ.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J24817
BBa_J24817 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters

BBa_J24818
BBa_J24818 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters

BBa_J24819
BBa_J24819 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters with J23101

BBa_J24820
BBa_J24820 Version 1 (Component)
Renilla luciferase reporter device for DLARAP

BBa_K249029
BBa_K249029 Version 1 (Component)
rpsA TIR (Translation Initiation Region)

BBa_K173013
BBa_K173013 Version 1 (Component)
pdc translational unit (RBS B0030)