BBa_K1065309
BBa_K1065309 Version 1 (Component)
light regulated circuit producing amilGFP+EFE at dark

BBa_K137046
BBa_K137046 Version 1 (Component)
150 bp inverted tetR promoter

BBa_M36000
BBa_M36000 Version 1 (Component)
5' Bicistronic UTR (weak), does not include ATG start

BBa_I20222
BBa_I20222 Version 1 (Component)
4B RBS from T7 charactization device

SspDnaB(N)
BBa_K1362408 Version 1 (Component)
SspDnaB N-Intein cloning piece

BBa_K2116095
BBa_K2116095 Version 1 (Component)
Pconst with 3 esaboxes and 8bp spacers

BBa_K1859010
BBa_K1859010 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*3 linker

BBa_K1859009
BBa_K1859009 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS*3 linker

BBa_K1859007
BBa_K1859007 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS*2 linker

BBa_K1859008
BBa_K1859008 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*2 linker

BBa_I745016
BBa_I745016 Version 1 (Component)
pLV-UBC-GFP-siGata3-5'

BBa_K1595028
BBa_K1595028 Version 1 (Component)
MBP with EcnB from Agrobacterium

BBa_B2022
BBa_B2022 Version 1 (Component)
4B RBS from T7

BBa_J23201
BBa_J23201 Version 1 (Component)
Ser2-tRNA key with 5bp spacer

BBa_J23202
BBa_J23202 Version 1 (Component)
Ser2-tRNA key with 7bp spacer

BBa_J23203
BBa_J23203 Version 1 (Component)
Ser2-tRNA key with 9bp spacer

CircNpu
BBa_K1362000 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct

O5
BBa_K2054005 Version 1 (Component)
Oligo 5 of DNA Tetrahedral Nanostructure

BBa_K861169
BBa_K861169 Version 1 (Component)
Indirect regulatory device, activated at high glucose concentration

BBa_K1332008
BBa_K1332008 Version 1 (Component)
mRNA circularization device (5?? side)

BBa_I13571
BBa_I13571 Version 1 (Component)
5' Hairpin RFP w Terminator

BBa_M11003
BBa_M11003 Version 1 (Component)
Wine Grape pyrroline-5-carboxylate synthetase

BBa_J58120
BBa_J58120 Version 1 (Component)
OmpR-P detector with a RFP pulse generator at intermediate concentrations

BBa_K1903019
BBa_K1903019 Version 1 (Component)
Intein N SSp DnaB S1

BBa_J176084
BBa_J176084 Version 1 (Component)
Polycomb ATF neg. ctrl. 5 "

BBa_J176074
BBa_J176074 Version 1 (Component)
Polycomb ATF neg. ctrl. 5

BBa_I13576
BBa_I13576 Version 1 (Component)
pR + RFP with 5' hairpin.

BBa_J176146
BBa_J176146 Version 1 (Component)
Test miR-luc sensor 5

BBa_I13572
BBa_I13572 Version 1 (Component)
Ptet + RFP with 5' hairpin.

BBa_M36361
BBa_M36361 Version 1 (Component)
5' Bicistronic UTR (apFAB866, GENE10_BCD2), No ATG Start

BBa_K1641004
BBa_K1641004 Version 1 (Component)
Fusion protein of Cre::EGFP::ssra-tag, with RBS at beginning

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

BBa_K1150051
BBa_K1150051 Version 1 (Component)
Truncated CMV dCas9 Device #5

BBa_K1150056
BBa_K1150056 Version 1 (Component)
Truncated SV40 dCas9 Device #5

BBa_J331014
BBa_J331014 Version 1 (Component)
Family 5 extracellular solute-binding protein gene sequence

BBa_J04606
BBa_J04606 Version 1 (Component)
Antiswitch repressing Biobrick coding sequence at the RBS ("Off" switch)

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

BBa_K1314018
BBa_K1314018 Version 1 (Component)
nif structure 5 in pUC57 (amp resistance)

BBa_K771308
BBa_K771308 Version 1 (Component)
FabI with Membrane Anchor 5(without VVD )

BBa_K771204
BBa_K771204 Version 1 (Component)
VioC with Membrane Anchor 5(without VVD)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

BBa_K426018
BBa_K426018 Version 1 (Component)
Pbad{rbs_pelB>},tn5,{NIS!}b1006Tn5 5

BBa_K1931013
BBa_K1931013 Version 1 (Component)
T7-RBS-Epidermicin NI01-MBP-Terminator

BBa_K1931014
BBa_K1931014 Version 1 (Component)
T7-RBS-Microcin E492-MBP-Terminator

BBa_K1150061
BBa_K1150061 Version 1 (Component)
Truncated dCas9 VP16 Device #5

BBa_M36080
BBa_M36080 Version 1 (Component)
Wild-type miraculin protein with His-tag at C-terminus

BBa_K1051974
BBa_K1051974 Version 1 (Component)
clb6 P + D-box + GFP + MER2 Intron + TOM40 + T

BBa_J54254
BBa_J54254 Version 1 (Component)
LacI_regulated LVA(B-5-1)

BBa_K137058
BBa_K137058 Version 1 (Component)
GFP fimE switch with 250 bp tetR promoter