BBa_K515107
BBa_K515107 Version 1 (Component)
Composite p(tetR) - Dendra2

BBa_K650007
BBa_K650007 Version 1 (Component)
ruvA (E. coli)

BBa_K650008
BBa_K650008 Version 1 (Component)
ruvB (E. coli)

BBa_K650009
BBa_K650009 Version 1 (Component)
radA (E. coli)

BBa_K650010
BBa_K650010 Version 1 (Component)
osmC (E. coli)

BBa_K650011
BBa_K650011 Version 1 (Component)
Tpx (E. coli)

BBa_K606049
BBa_K606049 Version 1 (Component)
Lambda phage attL recombination site

BBa_K606056
BBa_K606056 Version 1 (Component)
Double terminator + Lambda phage attR recombination site

BBa_K606061
BBa_K606061 Version 1 (Component)
SpoVG Ribosome Binding Site (RBS) for B. subtilis

BBa_K676011
BBa_K676011 Version 1 (Component)
Gyrase Binding Site from pSC101

BBa_K676012
BBa_K676012 Version 1 (Component)
Gyrase Binding Site from pBR322

BBa_K660500
BBa_K660500 Version 1 (Component)
Multiple Cloning Site (MCS)

ssTorA_CS-
BBa_K627013 Version 1 (Component)
Fusion of ssTorA, cleavage site of 14_3C protease and b-lactamase

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

scar
BBa_G0003 Version 1 (Component)
NheI/XbaI mixed site

scar
BBa_G0004 Version 1 (Component)
SpeI/NheI mixed site

BBa_K654059
BBa_K654059 Version 1 (Component)
Fatty Acyl-CoA Synthetase (Ligase) (fadD from E. coli) with 8-His Tag

BBa_K615004
BBa_K615004 Version 1 (Component)
Zif268 binding site-His3-URA3 construct for one-hybrid selection

BBa_K896003
BBa_K896003 Version 1 (Component)
Sulfide quinone reductase(SQR) on neutral site 2 vector

BBa_J72210
BBa_J72210 Version 1 (Component)
Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site

BBa_K817016
BBa_K817016 Version 1 (Component)
parS site

BBa_K817017
BBa_K817017 Version 1 (Component)
pSB2K3 with parS site

BBa_K737020
BBa_K737020 Version 1 (Component)
Cyanobacteria gene ribosome binding site

LipA
BBa_K836000 Version 1 (Component)
lipA from B. cepacia (codon usage optimized for E. coli)

lipA
BBa_K836003 Version 1 (Component)
lipA from B. cepacia (codon usage optimized for E. coli)

BBa_K737037
BBa_K737037 Version 1 (Component)
J23106,P0412,R0011,ClaI site,SalI site,truncated Spot42

BBa_K737057
BBa_K737057 Version 1 (Component)
Spot42 have a weak Hfq binding site(whereas galK weaker),an endogenous terminator(unclear efficiency

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K165030
BBa_K165030 Version 1 (Component)
mCYC promoter plus Zif268-HIV binding sites

BBa_K165031
BBa_K165031 Version 1 (Component)
mCYC promoter plus LexA binding sites

BBa_K165032
BBa_K165032 Version 1 (Component)
mCYC promoter plus Gli1 binding sites

BBa_K112121
BBa_K112121 Version 1 (Component)
BglII endonuclease with ribosome binding site

BBa_K165033
BBa_K165033 Version 1 (Component)
YY1 binding sites + mCYC promoter

BBa_K165038
BBa_K165038 Version 1 (Component)
Gli1 binding sites + ADH1 constitutive yeast promoter

BBa_K165039
BBa_K165039 Version 1 (Component)
Zif268-HIV binding sites + ADH1 yeast promoter

BBa_K165040
BBa_K165040 Version 1 (Component)
Gli1 binding sites + TEF constitutive yeast promoter

BBa_K165041
BBa_K165041 Version 1 (Component)
Zif268-HIV binding sites + TEF constitutive yeast promoter

BBa_K165042
BBa_K165042 Version 1 (Component)
Gli1 binding sites + MET25 inducible yeast promoter

BBa_K165043
BBa_K165043 Version 1 (Component)
Zif268-HIV binding sites + MET25 constitutive yeast promoter

BBa_K165045
BBa_K165045 Version 1 (Component)
pGAL1+ LexA bindingsites

BBa_K089005
BBa_K089005 Version 1 (Component)
-35 to Tc start site of phaC

BBa_K089006
BBa_K089006 Version 1 (Component)
-663 to Tc start site of phaC

BBa_K122021
BBa_K122021 Version 1 (Component)
Cassette3: Constitutive TAL with integration site

BBa_K116012
BBa_K116012 Version 1 (Component)
Ribosomal binding site + yaaU

BBa_K116013
BBa_K116013 Version 1 (Component)
Lac promoter + ribosomal binding site + yaaU

BBa_K112142
BBa_K112142 Version 1 (Component)
attR2 recombination site-reverse orientation

BBa_K112141
BBa_K112141 Version 1 (Component)
attR2 recombination site

BBa_J70032
BBa_J70032 Version 1 (Component)
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)