BBa_K199001
BBa_K199001 Version 1 (Component)
CUAGU tRNA Suppressor (Produces Serine)

BBa_J100063
BBa_J100063 Version 1 (Component)
this is a dummy part

BBa_K2110014
BBa_K2110014 Version 1 (Component)
MHETase enzyme after codon optimization for bacillus subtilis

BBa_K2110013
BBa_K2110013 Version 1 (Component)
PETase enzyme after codon optimization for bacillus subtilis

BBa_K566023
BBa_K566023 Version 1 (Component)
pcyA gene (constitutive expression)

BBa_K1453407
BBa_K1453407 Version 1 (Component)
Enzyme USB(AarI)-TAL USB-His Tag

BBa_K1453406
BBa_K1453406 Version 1 (Component)
Enzyme USB(BsmAI)-TAL USB-His Tag

mCherry
BBa_K165004 Version 1 (Component)
mCherry, yeast optimized for fusion

BBa_J120020
BBa_J120020 Version 1 (Component)
CH1 domain of human antibody

BBa_K1508002
BBa_K1508002 Version 1 (Component)
IIA(Glc) - E.coli glucose-specific phosphotransferase enzyme IIA

BBa_K1361006
BBa_K1361006 Version 1 (Component)
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter

BBa_I737000
BBa_I737000 Version 1 (Component)
from the workshop

BBa_J14467
BBa_J14467 Version 1 (Component)
Composite part comprised of I6032 and I13601

BBa_J14466
BBa_J14466 Version 1 (Component)
Composite part comprised of I6031 and I13600

BBa_K1607011
BBa_K1607011 Version 1 (Component)
The SCFV of anti-p185her2/neu antibody chA21 linked with EGFP by a (Gly4Ser)3 linker

<- BsaI
BBa_K1362423 Version 1 (Component)
BsaI reverse restriction site for RFC[105] cloning

BBa_K1132037
BBa_K1132037 Version 1 (Component)
AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter

BBa_K210003
BBa_K210003 Version 1 (Component)
Ura3. This is maker protein for yeast.

BBa_K1361002
BBa_K1361002 Version 1 (Component)
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter

BBa_K2184000
BBa_K2184000 Version 1 (Component)
Classical Cas9 endonuclease for knock-out of target genes

BBa_J120029
BBa_J120029 Version 1 (Component)
Composite part consisting of yeast promoter, CH1 domain of human antibody and GFP.

BBa_K218017
BBa_K218017 Version 1 (Component)
LuxO D47E under constitutive expression of TetR repressible promoter

BBa_K218021
BBa_K218021 Version 1 (Component)
LuxO D47A under constitutive expression of tetR repressible promoter

BBa_K1472611
BBa_K1472611 Version 1 (Component)
IPTG inducible expression of &#8710;9, &#8710;12, and &#8710;15 desaturases

BBa_K1585320
BBa_K1585320 Version 1 (Component)
GlgAB for polycistronic expression

BBa_K1431503
BBa_K1431503 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431501
BBa_K1431501 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431502
BBa_K1431502 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K209477
BBa_K209477 Version 1 (Component)
AarI B-C part, DOR

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K327014
BBa_K327014 Version 1 (Component)
The initiator

BBa_K1878001
BBa_K1878001 Version 1 (Component)
iRFP713 (the fluorescent protein formerly known as "iRFP")

BBa_M39203
BBa_M39203 Version 1 (Component)
Vector for creation of micro-dystrophin SFV

BBa_K581000
BBa_K581000 Version 1 (Component)
sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)

BBa_K726008
BBa_K726008 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_K1933103
BBa_K1933103 Version 1 (Component)
constitutive expression of CBDclos fused to BclA with 6xHis tag

BBa_K1933100
BBa_K1933100 Version 1 (Component)
constitutive expression of CBDcex fused to INPNC with 6xHis tag

BBa_K1933102
BBa_K1933102 Version 1 (Component)
constitutive expression of CBDcex fused to BclA with 6xHis tag

BBa_K1933101
BBa_K1933101 Version 1 (Component)
constitutive expression of CBDclos fused to INPNC with 6xHis tag

BBa_J70570
BBa_J70570 Version 1 (Component)
J04430 without the terminator BBa_J70570(AK)