BBa_K2082001
BBa_K2082001 Version 1 (Component)
Nanobody: Constant regions (insert variable regions to create your defined Nanobody or library)

BBa_K1987000
BBa_K1987000 Version 1 (Component)
GFP with BsaI sites for promoter insertion

BBa_K1994041
BBa_K1994041 Version 1 (Component)
sgRNA with dCas9 binding site Sequence 3 with PP7 handle insert

BBa_K1994042
BBa_K1994042 Version 1 (Component)
sgRNA with dCas9 binding site Sequence 6 and PP7 inserted

dCas9 B
BBa_K1903002 Version 1 (Component)
dCas9 with protein insertion frames version B

dCas9 A
BBa_K1903001 Version 1 (Component)
dCas9 with protein insertion frames version A

Insertion
BBa_K1903020 Version 1 (Component)
Insertion 1 for dCas9 version A

Insertion
BBa_K1903021 Version 1 (Component)
Insertion 2 for dCas9 version A

BBa_K1903022
BBa_K1903022 Version 1 (Component)
Insertion 1 for dCas9 version B

BBa_K1903023
BBa_K1903023 Version 1 (Component)
Insertion 2 for dCas9 version B

BBa_J70657
BBa_J70657 Version 1 (Component)
N-terminal 6 his fusion test for GFP J70610w604 inserts

BBa_J70659
BBa_J70659 Version 1 (Component)
N-terminal 6 strep fusion test for GFP J70610w n-terminalstreptag inserts

BBa_J70660
BBa_J70660 Version 1 (Component)
J70577 cut with BsaXI and with 6his inserts (J70564-fi) added

BBa_J70662
BBa_J70662 Version 1 (Component)
Short fusion insert test, designed from J70590 to recreate J04430

BBa_J100000
BBa_J100000 Version 1 (Component)
Cre with 8bp restriction sites and 1-Clause 2-SAT Problem Inserted

BBa_J119009
BBa_J119009 Version 1 (Component)
pLacI-RBS-T7RNAP with sites for oligo insertion

BBa_J100028
BBa_J100028 Version 1 (Component)
placeholder insert for BsaI Golden Gate Assembly of promoter

BBa_K590010
BBa_K590010 Version 1 (Component)
pGA1A3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

BBa_K590011
BBa_K590011 Version 1 (Component)
pGA1C3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

BBa_K590012
BBa_K590012 Version 1 (Component)
pGA4C5, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

BBa_K590013
BBa_K590013 Version 1 (Component)
pGA4A5, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

BBa_K590014
BBa_K590014 Version 1 (Component)
pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

pINDEL
BBa_K551001 Version 1 (Component)
plasmid of insertion and deletion

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Transfecti
BBa_K157041 Version 1 (Component)
Transfection vector (similar to pSB1AK8)

BBa_S04147
BBa_S04147 Version 1 (Component)
The Partial Butanol Operon (5 inserts from I725021 to I725025)

BBa_K112245
BBa_K112245 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid

BBa_K112246
BBa_K112246 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid

BBa_K112247
BBa_K112247 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid

BBa_K112248
BBa_K112248 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid

BBa_K127003
BBa_K127003 Version 1 (Component)
Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator

BBa_K106701
BBa_K106701 Version 1 (Component)
pCR2.1 TOPO Vector (to contain AarI inserts)

AD default
BBa_K106400 Version 1 (Component)
Default insert for AarI AD acceptor vectors

BBa_J70036
BBa_J70036 Version 1 (Component)
A BioScaffold Part (Uses AarI) for Library Insert Preparation (see Part Design page)

BBa_J70050
BBa_J70050 Version 1 (Component)
Left Library Insert Preparation Part (To use with J70052, and J70040)

BBa_J70052
BBa_J70052 Version 1 (Component)
Right Library Insert Preparation Part (To use with J70050, and J70040)

BBa_J70120
BBa_J70120 Version 1 (Component)
Library Insert Test Plasmid for J70050 and J70052

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_J70320
BBa_J70320 Version 1 (Component)
YFP, terminator insert position, mcherry

BBa_J70321
BBa_J70321 Version 1 (Component)
mcherry, terminator insert position, YFP

BBa_J70322
BBa_J70322 Version 1 (Component)
YFP, terminator insert position (bs part)

BBa_J70323
BBa_J70323 Version 1 (Component)
terminator insert position, YFP

BBa_J70347
BBa_J70347 Version 1 (Component)
GFP, terminator insert position

BBa_J70348
BBa_J70348 Version 1 (Component)
terminator insert position, GFP

BBa_J70349
BBa_J70349 Version 1 (Component)
promoter, GFP, terminator insert position, mcherry

BBa_J70350
BBa_J70350 Version 1 (Component)
promoter, mcherry, terminator insert position, GFP

BBa_K157045
BBa_K157045 Version 1 (Component)
Transfection vector insert (CMV.CFP)

BBa_J70604
BBa_J70604 Version 1 (Component)
J70589 cut with BsaXI and with rbs, 6his inserts (J70559-f1i,f2i,r2i,r2i) added

BBa_J70605
BBa_J70605 Version 1 (Component)
J70590 cut with BsaXI and with GSGSGS inserts (J70556-fi,ri) added