BBa_S01533
BBa_S01533 Version 1 (Component)
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BBa_S01453
BBa_S01453 Version 1 (Component)
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BBa_S01449
BBa_S01449 Version 1 (Component)
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BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_J72118
BBa_J72118 Version 1 (Component)
p15A assembly vector, pBjh1601CA

BBa_J72112
BBa_J72112 Version 1 (Component)
SpecR split R6K vector

BBa_J72111
BBa_J72111 Version 1 (Component)
SpecR split ColE2 vector

BBa_K911010
BBa_K911010 Version 1 (Component)
Bacillus shuttle vector (PJS130)

BBa_K112950
BBa_K112950 Version 1 (Component)
BBb1 entry vector - specR

BBa_K890000
BBa_K890000 Version 1 (Component)
methanococcus shuttle vector pAW42

BBa_J72110
BBa_J72110 Version 1 (Component)
AmpR/CamR p15A vector

BBa_K1363610
BBa_K1363610 Version 1 (Component)
key-1-of-or of RNA logic gates

BBa_K617003
BBa_K617003 Version 1 (Component)
R6K origin of replication

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

BBa_J64983
BBa_J64983 Version 1 (Component)
Strong OmpR Binding Site

BBa_J64986
BBa_J64986 Version 1 (Component)
LacI Consensus Binding Site

frr
BBa_K1668002 Version 1 (Component)
frr (from Streptomyces avermitilis, increasing avermectin production)

Pxyl
BBa_K143014 Version 1 (Component)
Promoter Xyl for B.subtilis

BBa_M1678
BBa_M1678 Version 1 (Component)
a construct to detect the presence/absence of glucose

BBa_K209413
BBa_K209413 Version 1 (Component)
AarI C-D part, Vav

BBa_K1689006
BBa_K1689006 Version 1 (Component)
Coding sequence of FKBP-Cluc394

BBa_K341457
BBa_K341457 Version 1 (Component)
Chloramphenicol resistance(with Promoter) + I-sceI site+ Recombination site

P43
BBa_K143013 Version 1 (Component)
Promoter 43 a constitutive promoter for B. subtilis

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_S01935
BBa_S01935 Version 1 (Component)
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BBa_S01985
BBa_S01985 Version 1 (Component)
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BBa_S01988
BBa_S01988 Version 1 (Component)
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BBa_S01839
BBa_S01839 Version 1 (Component)
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BBa_S01417
BBa_S01417 Version 1 (Component)
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BBa_K799024
BBa_K799024 Version 1 (Component)
pSB1C3 yGG promoter acceptor vector

BBa_J05217
BBa_J05217 Version 1 (Component)
Regulator for R2-YAP7

Altered Sequence Activities
AlteredSequenceActivities_collection Version 1 (Collection)
A set of activities to point to ways in which sequences have been altered between source and upload

Pnot(O55)
BBa_I732205 Version 1 (Component)
NOT Gate Promoter Family Member (D001O55)

phi80 attP
BBa_K300991 Version 1 (Component)
phi80 phage attP integration site

BBa_K145152
BBa_K145152 Version 1 (Component)
Hybrid promoter: P22 c2 , LacI NOR gate

BBa_I728978
BBa_I728978 Version 1 (Component)
Promoter+RBS+CPX(Circularly-Permuted OmpX for Surface Display of Polypeptides)

BBa_K1689005
BBa_K1689005 Version 1 (Component)
Coding sequence of FKBP-Cluc398

BBa_K1689002
BBa_K1689002 Version 1 (Component)
Coding sequence of Cluc-STV

BBa_M36124
BBa_M36124 Version 1 (Component)
Glucose-6-Phosphate Isomerase for manipulation of glycolysis in yeast

BBa_K2110015
BBa_K2110015 Version 1 (Component)
The key gene for accumulation of PHA in P.putida KT2440

BBa_K2110016
BBa_K2110016 Version 1 (Component)
The key gene for utilization of ethylene glycolin P.putida KT2440.

BBa_K143003
BBa_K143003 Version 1 (Component)
5??? Integration Sequence for the PyrD locus of B. subtilis

BBa_K349004
BBa_K349004 Version 1 (Component)
RFP BbsI BA (for Construction of AB Type Bytes-v2)

P-alsT
BBa_K818300 Version 1 (Component)
Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.

BBa_S01986
BBa_S01986 Version 1 (Component)
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BBa_S01933
BBa_S01933 Version 1 (Component)
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BBa_S01987
BBa_S01987 Version 1 (Component)
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BBa_K2074016
BBa_K2074016 Version 1 (Component)
*FMDV 2A(Non-functional) forChlamydomonas reinhardtii

BBa_S01641
BBa_S01641 Version 1 (Component)
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