BBa_K2107010
BBa_K2107010 Version 1 (Component)
Platelet Derived Growth Factor-B Isoform 1 (Serine Modified)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K782046
BBa_K782046 Version 1 (Component)
HindIII/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vecto

BBa_K799025
BBa_K799025 Version 1 (Component)
pSB1C3 yGG ORF acceptor vector

BBa_J120001
BBa_J120001 Version 1 (Component)
DFFb (CAD)-coding sequence for H. sapien DNA fragmentation factor beta

BBa_K2152001
BBa_K2152001 Version 1 (Component)
human Epidermal Growth Factor（hEGF） with FLAG-tag

BBa_K299509
BBa_K299509 Version 1 (Component)
Expression Vector pT7+B0034

BBa_K799026
BBa_K799026 Version 1 (Component)
pSB1C3 yGG transcriptional terminator acceptor vector

BBa_J120025
BBa_J120025 Version 1 (Component)
TACE(Tumor necrosis factor-α-converting enzyme) aka ADAM17 endopepsidase (H. sapiens)

BBa_K1734005
BBa_K1734005 Version 1 (Component)
C6 (hr5 enhancer + IE1 promoter + HBM signal sequence + Nanoluc + T2A + mRFP1 + T2A + Zeo Resistance

BBa_J95039
BBa_J95039 Version 1 (Component)
plasmid vector for Rhodobacter sphaeroides

BBa_J01120
BBa_J01120 Version 1 (Component)
Sacrafical OnRFP Kan vector

BBa_K109551
BBa_K109551 Version 1 (Component)
TrbA transcription factor for the Tra2 operon in incP alpha conjugation

BBa_K112995
BBa_K112995 Version 1 (Component)
BBb1 assembly vector - C/A

BBa_K1531005
BBa_K1531005 Version 1 (Component)
Bovine kappa casein (B variant) with full alpha-factor yeast secretion signal

BBa_K1531004
BBa_K1531004 Version 1 (Component)
Bovine beta casein (B variant) with full alpha-factor yeast secretion signal

BBa_K272501
BBa_K272501 Version 1 (Component)
TetR repressible promoter-Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance ma

BBa_K1172913
BBa_K1172913 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO Barnase)

BBa_K1657000
BBa_K1657000 Version 1 (Component)
It is called BAT. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1351040
BBa_K1351040 Version 1 (Component)
pBS0K<i>Pspac</i>, an IPTG-inducible replicative expression vector for

BBa_J72117
BBa_J72117 Version 1 (Component)
BBb High copy entry vector, pBca1256

BBa_K1172912
BBa_K1172912 Version 1 (Component)
Part 1 of the Biosystem TetOR alive (pRha TetR alr)

BBa_K1657002
BBa_K1657002 Version 1 (Component)
It is called TAB. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1657001
BBa_K1657001 Version 1 (Component)
It is called BAT. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1657004
BBa_K1657004 Version 1 (Component)
It is called TAB. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1657003
BBa_K1657003 Version 1 (Component)
It is called BAT. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K2005051
BBa_K2005051 Version 1 (Component)
mCherry with T7 expression (oxidation-resistant)

BBa_K1172914
BBa_K1172914 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO GFP)

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

BBa_M36745
BBa_M36745 Version 1 (Component)
The sensor codes for AraC, a transcription factor, which represses the operon.Arabinose induces tran

BBa_K106693
BBa_K106693 Version 1 (Component)
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

BBa_M36475
BBa_M36475 Version 1 (Component)
Codes for AraC, a transcription factor which represses operon. Arabinose dislodges TF protein.

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;24 followed by YFP

BBa_K086008
BBa_K086008 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;28 followed by YFP

BBa_K086006
BBa_K086006 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;28 followed by YFP

BBa_K086005
BBa_K086005 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;28 followed by YFP