CBDcex
BBa_K863101 Version 1 (Component)
Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)

BBa_K1077003
BBa_K1077003 Version 1 (Component)
J23100 fim switch b0034 GFP

BBa_M31173
BBa_M31173 Version 1 (Component)
M13.1 from HpaI to BamHI, for reals this time!

BBa_K1159009
BBa_K1159009 Version 1 (Component)
Secretory Erythromycin Esterase Type II (SERK-SigP_EreB) in RFC[25] N-Part

BBa_M36994
BBa_M36994 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat)

BBa_M36991
BBa_M36991 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat)

BBa_M36993
BBa_M36993 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (10-Repeat)

BBa_M36990
BBa_M36990 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (5-Repeat)

BBa_M36995
BBa_M36995 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (5-Repeat)

BBa_M11406
BBa_M11406 Version 1 (Component)
Type 1 promoter of psaAB operon in Synechocystis sp. PCC 6803

BBa_M11405
BBa_M11405 Version 1 (Component)
Type 1 promoter of petBD operon in Synechocystis sp. PCC 6803

BBa_M45138
BBa_M45138 Version 1 (Component)
Type II Secretion of Chromate and Uranium Reductase with TorA

BBa_K2041002
BBa_K2041002 Version 1 (Component)
FimE-T

BBa_K905000
BBa_K905000 Version 1 (Component)
nirB promoter, it will be induced in anaerobic enviroment

BBa_K880001
BBa_K880001 Version 1 (Component)
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K

BBa_K1405007
BBa_K1405007 Version 1 (Component)
A Kill Switch with "memory" time repressed by IPTG

BBa_K1159014
BBa_K1159014 Version 1 (Component)
Membrane-anchored Erythromycin Esterase Type II in RFC[25] N-Part

BBa_M36983
BBa_M36983 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (5-Repeat) Correct

BBa_M36982
BBa_M36982 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat) Correct

BBa_M11403
BBa_M11403 Version 1 (Component)
Type 1 promoter of the cpcBA operon in Synechocystis sp. PCC 6803

BBa_K1632002
BBa_K1632002 Version 1 (Component)
fim switch[default ON](Tokyo_Tech/J23119)_rbs_gfp

BBa_K1405008
BBa_K1405008 Version 1 (Component)
A Kill Switch with "memory" time repressed by IPTG

BBa_J58011
BBa_J58011 Version 1 (Component)
Promoter which is activated by cI and CRP, using a transcription logic function type AND

BBa_K1077006
BBa_K1077006 Version 1 (Component)
nat fim switch b0034 GFP OFF orientation

BBa_K1947020
BBa_K1947020 Version 1 (Component)
This polypeptide will be fused with the recombinant protein which we want to purify.

BBa_K1632030
BBa_K1632030 Version 1 (Component)
fim switch[default ON](Tokyo_Tech/J23119)_rbs_gfp_rbs_rhlI

BBa_K1632032
BBa_K1632032 Version 1 (Component)
fim switch[default ON](Tokyo_Tech/J23119)_rbs_gfp_rbs_lasI

BBa_K1632031
BBa_K1632031 Version 1 (Component)
fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_rhlI

BBa_K1632033
BBa_K1632033 Version 1 (Component)
fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_lasI

BBa_K1077007
BBa_K1077007 Version 1 (Component)
J23100 fim switch b0034 amilCP ON orientation

BBa_K758003
BBa_K758003 Version 1 (Component)
UAS, this part consists of five UAS sequences and hsp70 TATA.

BBa_K1159005
BBa_K1159005 Version 1 (Component)
Secretory Erythromycin Esterase Type II (IgKappa-SigP_EreB) in RFC[25] N-Part

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K1487031
BBa_K1487031 Version 1 (Component)
Promoter + Guide RNA (gRNA) target for tcpE

BBa_M45091
BBa_M45091 Version 1 (Component)
AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa

BBa_K1487073
BBa_K1487073 Version 1 (Component)
eYFP Reporter for tcpE gRNA target with strong promoter.

BBa_K377704
BBa_K377704 Version 1 (Component)
FimB

BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

BBa_J64701
BBa_J64701 Version 1 (Component)
TrpE

BBa_I729009
BBa_I729009 Version 1 (Component)
Time device

BBa_K1369007
BBa_K1369007 Version 1 (Component)
pBAD FsrA-SH3pep, pFsrA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations wil

CapD
BBa_K314970 Version 1 (Component)
this is a really long short description. Will it still go through? this is a really long short descr

BBa_K187416
BBa_K187416 Version 1 (Component)
trpE in pAB

BBa_M11407
BBa_M11407 Version 1 (Component)
Type 1 promoter of hspA gene in Synechocystis sp. PCC 6803

BBa_M11408
BBa_M11408 Version 1 (Component)
Type 1 promoter of sigA gene in Synechocystis sp. PCC 6803

BBa_M11404
BBa_M11404 Version 1 (Component)
Type 1 promoter of psbA2 gene in Synechocystis sp. PCC 6803

BBa_K2052016
BBa_K2052016 Version 1 (Component)
FimH site directed mutated with RPMrel and ButCoat

BBa_M11411
BBa_M11411 Version 1 (Component)
Type 2 promoter of lrtA gene. Gene is expressed during darkness. Potentially darkness-induced promot

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.