BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

BBa_K931003
BBa_K931003 Version 1 (Component)
An L-Arabinose Controlled Vitamin B12 Binding - Starch Binding Protein Fusion

BBa_K078002
BBa_K078002 Version 1 (Component)
A fragment that encodes an enzyme involed in dioxin degradation, the third step

BBa_K132016
BBa_K132016 Version 1 (Component)
luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator

BBa_K2088006
BBa_K2088006 Version 1 (Component)
It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I

Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')

Devices from the iGEM 2016 interlab
iGEM_2016_interlab_collection Version 1 (Collection)
This is a collection of devices that were used in the 2016 iGEM interlab study

BBa_K2007003
BBa_K2007003 Version 1 (Component)
Constitutive promoter, an RBS, and Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_M45102
BBa_M45102 Version 1 (Component)
Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

YFP_SPfer
BBa_K809314 Version 1 (Component)
YFP + signal peptide of FER

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_K1985013
BBa_K1985013 Version 1 (Component)
Sequence coding for Sup35 with residues 1-61 with an arabinose inducible promoter

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_K189060
BBa_K189060 Version 1 (Component)
Gp15 of Bacteriophage Mu

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K1676120
BBa_K1676120 Version 1 (Component)
Mutant 16 of Lactate Dehydrogenase

BBa_K1676114
BBa_K1676114 Version 1 (Component)
Mutant 10 of Lactate Dehydrogenase

BBa_K557000
BBa_K557000 Version 1 (Component)
CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi

BBa_J70652
BBa_J70652 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K1228001
BBa_K1228001 Version 1 (Component)
A fragment of loctoferrin

BBa_K2007004
BBa_K2007004 Version 1 (Component)
Fatty acid sensitive promoter, an RBS, and Drosophila melanogaster Juvenile Hormone Acid O-methyltra

BBa_K1179017
BBa_K1179017 Version 1 (Component)
A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation

BBa_K2041010
BBa_K2041010 Version 1 (Component)
plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T

BBa_J70653
BBa_J70653 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_K1985014
BBa_K1985014 Version 1 (Component)
Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter

BBa_K809108
BBa_K809108 Version 1 (Component)
Efficiency test of Q0255 Terminator

BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter

RFP_SPtim2
BBa_K809303 Version 1 (Component)
RFP + signal peptide of TIM21

BBa_K337088
BBa_K337088 Version 1 (Component)
Fragment 7 of wt AAV6

BBa_K337060
BBa_K337060 Version 1 (Component)
Fragment 3 of wt AAV1

BBa_K1413042
BBa_K1413042 Version 1 (Component)
OriVR6Kgamma origin of replication (ori) 2

T25 domain
BBa_K1088056 Version 1 (Component)
T25 domain of CyaA from Bordetella pertussis

BBa_K648103
BBa_K648103 Version 1 (Component)
RecA with mutation of Arg 243

CsgD
BBa_K1019001 Version 1 (Component)
CsgD: positive regulator of curlin genes

BBa_K648102
BBa_K648102 Version 1 (Component)
RecA with mutation of Lys 286

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.