BBa_K2018036
BBa_K2018036 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_K2018033
BBa_K2018033 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_J04480
BBa_J04480 Version 1 (Component)
EYFP coding device with promoter, RBS, and terminator. No degradation tag.

BBa_K1897033
BBa_K1897033 Version 1 (Component)
superfolded Green Fluorescent Protein

BBa_M30012
BBa_M30012 Version 1 (Component)
OmpR controlled mRFP

BBa_K900003
BBa_K900003 Version 1 (Component)
Vacuolar Membrane Targeting Protein

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

BBa_K1162014
BBa_K1162014 Version 1 (Component)
protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon

BBa_I20292
BBa_I20292 Version 1 (Component)
There is no limit to what a man can do or where he can go if...

BBa_K242300
BBa_K242300 Version 1 (Component)
multi-Ler binding site +promoter 1

BBa_M45016
BBa_M45016 Version 1 (Component)
phasin protein

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1605030
BBa_K1605030 Version 1 (Component)
Ptet mRFP nifH RBS

BBa_K1605001
BBa_K1605001 Version 1 (Component)
Ptet mRFP nifZ RBS

BBa_K861160
BBa_K861160 Version 1 (Component)
CRP, cAMP receptor protein

BBa_K542012
BBa_K542012 Version 1 (Component)
Catechol 2,3-dioxygenase with a C-terminal Arg-tag and Double Terminator (no promoter/RBS)

BBa_K258016
BBa_K258016 Version 1 (Component)
Vhb protein

BBa_K1510017
BBa_K1510017 Version 1 (Component)
red florescence protein RFC23

BBa_K1051116
BBa_K1051116 Version 1 (Component)
Actin Targeting Protein

BBa_M1174
BBa_M1174 Version 1 (Component)
protein1

enhanced
BBa_K2075011 Version 1 (Component)
enhanced Green fluorescenc protein (GFP)

enhanced G
BBa_K2075012 Version 1 (Component)
enhanced Green fluorescenc protein (GFP)

BBa_K1123017
BBa_K1123017 Version 1 (Component)
DNA Coding Sequence for EGFP Protein

BBa_K648006
BBa_K648006 Version 1 (Component)
Long 10AA Fusion Protein Linker with Standard 25 Prefix/Suffix

BBa_J58009
BBa_J58009 Version 1 (Component)
Fusion protein Trg-EnvZ

BBa_J64863
BBa_J64863 Version 1 (Component)
Strong OmpR Binding Operon for Team Challenge 03-2007

BBa_K125550
BBa_K125550 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_K1014016
BBa_K1014016 Version 1 (Component)
HrpS Protein Generator

BBa_K1434002
BBa_K1434002 Version 1 (Component)
cAMP receptor protein (CRP) mutant

BBa_J133568
BBa_J133568 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133564
BBa_J133564 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133573
BBa_J133573 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133563
BBa_J133563 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133572
BBa_J133572 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133579
BBa_J133579 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_K360123
BBa_K360123 Version 1 (Component)
trpL promoter + RFP protein

BBa_J133566
BBa_J133566 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133570
BBa_J133570 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133569
BBa_J133569 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133578
BBa_J133578 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133580
BBa_J133580 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133565
BBa_J133565 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133562
BBa_J133562 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133581
BBa_J133581 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133567
BBa_J133567 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133571
BBa_J133571 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_K1947028
BBa_K1947028 Version 1 (Component)
Improve the protein purification.

BBa_K1947027
BBa_K1947027 Version 1 (Component)
Improve the protein purification.

BBa_K1947026
BBa_K1947026 Version 1 (Component)
Improve the protein purification.