BBa_S01907BBa_S01907 Version 1 (Component)Intermediate part from assembly 240
BBa_S01936BBa_S01936 Version 1 (Component)Intermediate part from assembly 240
BBa_S02107BBa_S02107 Version 1 (Component)Intermediate part from assembly 240
BBa_S01996BBa_S01996 Version 1 (Component)Intermediate part from assembly 240
BBa_S01376BBa_S01376 Version 1 (Component)Intermediate part from assembly 239
BBa_S01379BBa_S01379 Version 1 (Component)Intermediate part from assembly 239
BBa_S01663BBa_S01663 Version 1 (Component)Intermediate part from assembly 240
BBa_S01660BBa_S01660 Version 1 (Component)Intermediate part from assembly 240
BBa_S01898BBa_S01898 Version 1 (Component)Intermediate part from assembly 240
BBa_S01381BBa_S01381 Version 1 (Component)Intermediate part from assembly 239
BBa_S01830BBa_S01830 Version 1 (Component)Intermediate part from assembly 240
BBa_S01845BBa_S01845 Version 1 (Component)Intermediate part from assembly 240
BBa_S01380BBa_S01380 Version 1 (Component)Intermediate part from assembly 239
BBa_S01377BBa_S01377 Version 1 (Component)Intermediate part from assembly 239
BBa_S01842BBa_S01842 Version 1 (Component)Intermediate part from assembly 240
BBa_S01383BBa_S01383 Version 1 (Component)Intermediate part from assembly 239
BBa_S01843BBa_S01843 Version 1 (Component)Intermediate part from assembly 240
BBa_S01378BBa_S01378 Version 1 (Component)Intermediate part from assembly 239
BBa_S01826BBa_S01826 Version 1 (Component)Intermediate part from assembly 240
BBa_S01382BBa_S01382 Version 1 (Component)Intermediate part from assembly 239
BBa_S01821BBa_S01821 Version 1 (Component)Intermediate part from assembly 240
BBa_S02001BBa_S02001 Version 1 (Component)Intermediate part from assembly 240
LacYBBa_K079014 Version 1 (Component)LacY transporter protein from E. coli
BBa_K1725317BBa_K1725317 Version 1 (Component)Member of the RBS library - derived from BBa_B0032
BBa_K619894BBa_K619894 Version 1 (Component)mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K1447004BBa_K1447004 Version 1 (Component)Epitope 1-5 from Ara h 1
BBa_K1447005BBa_K1447005 Version 1 (Component)Epitope 1-5 from Ara h 1
BBa_K619895BBa_K619895 Version 1 (Component)mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K833002BBa_K833002 Version 1 (Component)mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K833006BBa_K833006 Version 1 (Component)Mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K833008BBa_K833008 Version 1 (Component)Mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K2066500BBa_K2066500 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
BBa_K1441013BBa_K1441013 Version 1 (Component)DNA ligase from Escherichia coli with His-tag INSERT
BBa_K1323020BBa_K1323020 Version 1 (Component)oriV from the Staphylococcus aureus pSK41 plasmid clone 3
BBa_K1497197BBa_K1497197 Version 1 (Component)B0034-CHI - Chalcone Isomerase from Petunia with strong RBS
BBa_K1465203BBa_K1465203 Version 1 (Component)Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechococcus elongatus
BBa_K1968014BBa_K1968014 Version 1 (Component)Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae
BBa_K294205BBa_K294205 Version 1 (Component)This is a coding sequence of heat shock protein from E.coli
BBa_K2092004BBa_K2092004 Version 1 (Component)alcR (incl RBS), ethanol-activated transcription factor from A. nidulans
BBa_K1968009BBa_K1968009 Version 1 (Component)PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
BBa_K1968013BBa_K1968013 Version 1 (Component)PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige
BBa_K177035BBa_K177035 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.