BBa_M36824
BBa_M36824 Version 1 (Component)
Zinc Sensor

BBa_K1124215
BBa_K1124215 Version 1 (Component)
pLux-mCherry-LVA

BBa_K106004
BBa_K106004 Version 1 (Component)
mCherry, Aar1 AB part

BBa_K1688007
BBa_K1688007 Version 1 (Component)
ModLac laccase with His-tag and HlyA export tag (inc RBS and J23110 promoter)

BBa_K887004
BBa_K887004 Version 1 (Component)
Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015

BBa_K1500016
BBa_K1500016 Version 1 (Component)
paroF->MCherry-MutH(E56A)

BBa_K1500014
BBa_K1500014 Version 1 (Component)
paroF->MCherry-MutH(E56A)

BBa_J69502
BBa_J69502 Version 1 (Component)
CI-repressible mCherry Reporter

BBa_K165090
BBa_K165090 Version 1 (Component)
Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS306

BBa_K874999
BBa_K874999 Version 1 (Component)
Zinc Finger Array v1

BBa_I731017
BBa_I731017 Version 1 (Component)
Ptet bicistronic 3OC6HSL Sender + mCherry

BBa_K165092
BBa_K165092 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Gli1 repressor (mCherryx2 tagged) on pRS303

BBa_K165095
BBa_K165095 Version 1 (Component)
Gli1 bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS303

BBa_I23000
BBa_I23000 Version 1 (Component)
RBS Scaffold with mCherry reporter

BBa_K165091
BBa_K165091 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS306

BBa_K1141000
BBa_K1141000 Version 1 (Component)
Plac-RBS-mCherry-double terminator (IPTG-inducible)

BBa_J69522
BBa_J69522 Version 1 (Component)
tetR-repressible promoter coupled with mCherry Reporter

BBa_K1033250
BBa_K1033250 Version 1 (Component)
mCherry fusion protein codon optimised for Lactobacillus reuteri

BBa_K639004
BBa_K639004 Version 1 (Component)
rrnB P1-LacI-pLac-mCherry plausible stress sensor

BBa_J06603
BBa_J06603 Version 1 (Component)
Construction Intermediate: mCherry, bacterial (LVA+) with RBS (B0034.J06505)

BBa_I2036
BBa_I2036 Version 1 (Component)
mCherry reporter device and GFP reporter device in sequence

BBa_I731014
BBa_I731014 Version 1 (Component)
The luxR based receiver, F2620 (formerly I13270), controls the production of mCherry

BBa_K415005
BBa_K415005 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term

BBa_K1606010
BBa_K1606010 Version 1 (Component)
Kumamax enzyme that gluten updated m-cherry reporter device

BBa_K323164
BBa_K323164 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5

BBa_K323163
BBa_K323163 Version 1 (Component)
VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5

BBa_K415011
BBa_K415011 Version 1 (Component)
PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

BBa_K165100
BBa_K165100 Version 1 (Component)
Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*

BBa_K165101
BBa_K165101 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.