BBa_K1109007
BBa_K1109007 Version 1 (Component)
FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence transc

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

BBa_J31016
BBa_J31016 Version 1 (Component)
part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t

BBa_K2088006
BBa_K2088006 Version 1 (Component)
It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I

Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')

BBa_J70560
BBa_J70560 Version 1 (Component)
GFP, with N-terminal 6 his

BBa_K1845001
BBa_K1845001 Version 1 (Component)
Miraculin (Yeast codon optimised + His-tag)

Devices from the iGEM 2016 interlab
iGEM_2016_interlab_collection Version 1 (Collection)
This is a collection of devices that were used in the 2016 iGEM interlab study

BBa_M45102
BBa_M45102 Version 1 (Component)
Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

YFP_SPfer
BBa_K809314 Version 1 (Component)
YFP + signal peptide of FER

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

BBa_K1732010
BBa_K1732010 Version 1 (Component)
pSB1C3-J23100-B0034-E0040-His-B0015

BBa_K987000
BBa_K987000 Version 1 (Component)
This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p

BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_K189060
BBa_K189060 Version 1 (Component)
Gp15 of Bacteriophage Mu

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K1676120
BBa_K1676120 Version 1 (Component)
Mutant 16 of Lactate Dehydrogenase

BBa_K1676114
BBa_K1676114 Version 1 (Component)
Mutant 10 of Lactate Dehydrogenase

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_K1228001
BBa_K1228001 Version 1 (Component)
A fragment of loctoferrin

BBa_K2041010
BBa_K2041010 Version 1 (Component)
plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T

BBa_K792000
BBa_K792000 Version 1 (Component)
Yeast exportable His-rich peptide w/enhanced import (nonStd)

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K809108
BBa_K809108 Version 1 (Component)
Efficiency test of Q0255 Terminator

BBa_K1974011
BBa_K1974011 Version 1 (Component)
T7 Promoter+RBS+Hv1a+linker+6X His-Tag

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_I715048
BBa_I715048 Version 1 (Component)
Hin + pT7 -> RFP1 + GFP1 medium initial orientation

BBa_I715050
BBa_I715050 Version 1 (Component)
Hin + pT7 -> RFP1 + GFP1 medium initial orientation

RFP_SPtim2
BBa_K809303 Version 1 (Component)
RFP + signal peptide of TIM21

BBa_K337088
BBa_K337088 Version 1 (Component)
Fragment 7 of wt AAV6

BBa_K337060
BBa_K337060 Version 1 (Component)
Fragment 3 of wt AAV1

BBa_K1413042
BBa_K1413042 Version 1 (Component)
OriVR6Kgamma origin of replication (ori) 2

T25 domain
BBa_K1088056 Version 1 (Component)
T25 domain of CyaA from Bordetella pertussis

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1351017
BBa_K1351017 Version 1 (Component)
SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>

BBa_M31786
BBa_M31786 Version 1 (Component)
1st half of Gene III- preBamHI cut

BBa_K648103
BBa_K648103 Version 1 (Component)
RecA with mutation of Arg 243