BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K143071
BBa_K143071 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB) (with CmR)

BBa_K143072
BBa_K143072 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG) (with CmR)

BBa_K143073
BBa_K143073 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)

BBa_K143074
BBa_K143074 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)

BBa_J69522
BBa_J69522 Version 1 (Component)
tetR-repressible promoter coupled with mCherry Reporter

Eukaryotic
BBa_K157040 Version 1 (Component)
Eukaryotic terminator vector (very similar to pSB1AK8)

Transfecti
BBa_K157041 Version 1 (Component)
Transfection vector (similar to pSB1AK8)

BBa_K156001
BBa_K156001 Version 1 (Component)
BioBrick Placeholder 1 (Prefix - ApoI (NotI) AvrII - Suffix)

BBa_K156002
BBa_K156002 Version 1 (Component)
BioBrick placeholder 2 (Prefix - ApoI (NotI) NheI - Suffix)

BBa_K156003
BBa_K156003 Version 1 (Component)
BioBrick placeholder 3 (Prefix - ApoI (NotI) NsiI - Suffix)

BBa_K156004
BBa_K156004 Version 1 (Component)
BioBrick placeholder 4 (Prefix - ApoI (NotI) SbfI - Suffix)

BBa_K156005
BBa_K156005 Version 1 (Component)
BioBrick placeholder 5 (Prefix - MfeI (NotI) AvrII - Suffix)

BBa_K156006
BBa_K156006 Version 1 (Component)
BioBrick placeholder 6 (Prefix - MfeI (NotI) NheI - Suffix)

BBa_K156007
BBa_K156007 Version 1 (Component)
BioBrick placeholder 7 (Prefix - MfeI (NotI) Nsil - Suffix)

BBa_K156008
BBa_K156008 Version 1 (Component)
BioBrick placeholder 8 (Prefix - MfeI (NotI) SbfI - Suffix)

BBa_K122016
BBa_K122016 Version 1 (Component)
4CL:STS with CYC1 terminator.

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

RBS-cI
BBa_K093006 Version 1 (Component)
cI repressor with RBS

oriTr/LacZ
BBa_K125350 Version 1 (Component)
oriTr with lac promoter and lacZ

oriT/LacZ
BBa_K125360 Version 1 (Component)
oriT with lac promoter and lacZ

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086018
BBa_K086018 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086019
BBa_K086019 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086020
BBa_K086020 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K106342
BBa_K106342 Version 1 (Component)
AB shuttle vector

BBa_K106343
BBa_K106343 Version 1 (Component)
BD shuttle vector

pBSEP1
BBa_K090401 Version 1 (Component)
Gram-positive Shuttle Vector for Episomal Expression

BBa_K142008
BBa_K142008 Version 1 (Component)
lacI IS mutant R197A with RBS and terminator

BBa_K142009
BBa_K142009 Version 1 (Component)
lacI IS mutant R197F with RBS and terminator

BBa_K142010
BBa_K142010 Version 1 (Component)
lacI IS mutant T276A with RBS and terminator

BBa_K142011
BBa_K142011 Version 1 (Component)
lacI IS mutant T276Fwith RBS and terminator

BBa_K142012
BBa_K142012 Version 1 (Component)
lacI IS mutant R197A T276A with RBS and terminator

BBa_K142013
BBa_K142013 Version 1 (Component)
lacI IS mutant R197A T276F with RBS and terminator

BBa_K142014
BBa_K142014 Version 1 (Component)
lacI IS mutant R197F T276A with RBS and terminator

BBa_K142015
BBa_K142015 Version 1 (Component)
lacI IS mutant R197F T276F with RBS and terminator

BBa_K142202
BBa_K142202 Version 1 (Component)
SceI with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K142203
BBa_K142203 Version 1 (Component)
T4 ligase with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K146000
BBa_K146000 Version 1 (Component)
pCR2.1 PCR product cloning vector

BBa_K106672
BBa_K106672 Version 1 (Component)
AarI AD acceptor vector (pRS305, Gal1P, Adh1t)

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K142025
BBa_K142025 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette