BBa_K587008
BBa_K587008 Version 1 (Component)
Crxst -> Lock sequence for low concentration mechanism

BBa_I735000
BBa_I735000 Version 1 (Component)
ech gene coding sequence for feruoyl CoA hydratese

BBa_K2184029
BBa_K2184029 Version 1 (Component)
SNP for non taster NaCl & Sour -TAS1R1 rs17492553

cln2
BBa_K105014 Version 1 (Component)
cln2 PEST destabilization domain for rapid protein turnover

BBa_J24818
BBa_J24818 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters

BBa_M50016
BBa_M50016 Version 1 (Component)
Promoter, RBS and GolS Activator for Gold Detection

BBa_K812132
BBa_K812132 Version 1 (Component)
mCFP with kozak sequence for expression in Xenopus

BBa_K541000
BBa_K541000 Version 1 (Component)
Limulus anti-LPS factor (LALF) for Bacillus subtilis

BBa_K812133
BBa_K812133 Version 1 (Component)
sfGFP with kozak sequence for expression in Xenopus

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_J329004
BBa_J329004 Version 1 (Component)
Quorum Sensing Sender Part for Use in BBa_J329003

BBa_K2123301
BBa_K2123301 Version 1 (Component)
Novel Synthetic Phytochelatin codon optimized for E. coli expression

KanR-BA-Bf
BBa_K349012 Version 1 (Component)
KanR-BA-BfuAI (for construction of BA BioBytes 2.0)

BBa_K812130
BBa_K812130 Version 1 (Component)
Citrine reporter with a Kozak sequence for expression in Xenopus

BBa_K812120
BBa_K812120 Version 1 (Component)
IaaH on PSC2+ Ready for use in xenopus tropicalis egg

BBa_K251000
BBa_K251000 Version 1 (Component)
coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)

BBa_K363002
BBa_K363002 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

Adapter Bi
BBa_K1807000 Version 1 (Component)
Protein generator device suitable for blue-white screening and Gibson Assembly.

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

BBa_K1942000
BBa_K1942000 Version 1 (Component)
A shRNA corresponding DNA sequence for KRAS which could silence the gene

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K2020051
BBa_K2020051 Version 1 (Component)
wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K2123116
BBa_K2123116 Version 1 (Component)
Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.