BBa_K1375022BBa_K1375022 Version 1 (Component)promoter+RBS+araC+ DT+pBAD+RBS+Cinr+RBS+CinI+DT+Plac+RBS+LuxI+DT+Pcon+RBS+LacI
BBa_S03362BBa_S03362 Version 1 (Component)LacI input to switch
BBa_K876041BBa_K876041 Version 1 (Component)TT-pLsrA-LacI
BBa_K2188095BBa_K2188095 Version 1 (Component)Mammalian LacI And Terminator
BBa_K105100BBa_K105100 Version 1 (Component)LacI - DNA binding domain
BBa_K1520024BBa_K1520024 Version 1 (Component)Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
BBa_K822004BBa_K822004 Version 1 (Component)LacI generator (no promoter)
BBa_I14002BBa_I14002 Version 1 (Component)LacI with strong RBS
BBa_J09340BBa_J09340 Version 1 (Component)lacI with medium RBS
BBa_K228802BBa_K228802 Version 1 (Component)RBS(B0032)+lacI(C0012)
BBa_K228803BBa_K228803 Version 1 (Component)RBS(B0034)+lacI(C0012)
BBa_K228801BBa_K228801 Version 1 (Component)RBS(B0030)+lacI(C0012)
BBa_K185031BBa_K185031 Version 1 (Component)rbs34-lacI intermediate part
BBa_K1421003BBa_K1421003 Version 1 (Component)pCl+RBS+LacI+2TE
BBa_K132005BBa_K132005 Version 1 (Component)kanR+lacI+Plac+kanR
BBa_K132006BBa_K132006 Version 1 (Component)kanR+lacI+Plac+kanR
BBa_K132004BBa_K132004 Version 1 (Component)kanR+lacI+Plac+kanR
BBa_K292002BBa_K292002 Version 1 (Component)pLac (LacI regulated) + Strong RBS
Plamda-LacBBa_I763010 Version 1 (Component)LacI repressor regulated by Plamda Promoter
BBa_K1713010BBa_K1713010 Version 1 (Component)J23100+B0034+lacI(-LVA)+B0015
BBa_J01108BBa_J01108 Version 1 (Component)TEST [pTet][StrRBS][LacI][DblTerm]
BBa_K1053302BBa_K1053302 Version 1 (Component)λPR-RBS-lacI-DT
BBa_K228813BBa_K228813 Version 1 (Component)RBS(B0034)+lacI(C0012)+terminator(B0015)
BBa_K318509BBa_K318509 Version 1 (Component)lacI pL + RBS + cI LVA + TT
RBS-LacI-TBBa_K079002 Version 1 (Component)LacI protein with terminator and RBS
BBa_K934002BBa_K934002 Version 1 (Component)LuxR/3OC6HSL complex dependent LacI generator
BBa_K546547BBa_K546547 Version 1 (Component)Constitutive (tetR repressible) LacI and RFP expression
BBa_K188122BBa_K188122 Version 1 (Component)RBS+lacI repressor from E. coli (+LVA)
BBa_K1321362BBa_K1321362 Version 1 (Component)sfGFP fused to CBDcex driven by LacI
BBa_K590046BBa_K590046 Version 1 (Component)AAR-PSB3K3-Lac Inducible w/o LacI
BBa_K876016BBa_K876016 Version 1 (Component)plac-lacO-RBS-GFP-T-pTet-LacI
BBa_K1472613BBa_K1472613 Version 1 (Component)∆9, ∆12, ∆15 desaturases with LacI promoter
BBa_K639004BBa_K639004 Version 1 (Component)rrnB P1-LacI-pLac-mCherry plausible stress sensor
BBa_K1615108BBa_K1615108 Version 1 (Component)mRFP fused to CBDclos driven by LacI promoter
BBa_J06916BBa_J06916 Version 1 (Component)Lambda cI QPI regulated by LacI with EYFP
BBa_K549004BBa_K549004 Version 1 (Component)LacI promotor fused with the iron dependent regulator fur
BBa_J47053BBa_J47053 Version 1 (Component)Constitutive device (medium transcription) for lacI repressor, strong RBS
BBa_K132016BBa_K132016 Version 1 (Component)luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
FBS-AceB+LBBa_K1163999 Version 1 (Component)Inverter composed of Fur Binding site from AceB promoter + LacI-LVA
BBa_K079051BBa_K079051 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator
BBa_K1520509BBa_K1520509 Version 1 (Component)PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.