| Types | DnaRegion
|
| Roles | Project
engineered_region
|
| Sequences | BBa_I3425_sequence (Version 1)
|
Description
Tests ability of switch to output aspartate given particular inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
Notes
The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the AspA attractant sender (I3410.I3200). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
