Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lejian Jiang
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-19 03:08:52
AHL,DPD double-cycled oscillation
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1886002_sequence (Version 1) |
Description
Two molecules of luxR binds with AHL (catalyzed by luxI), and this compound will promote the activity of pluxR. pluxR controls the expression of gene aiiA and luxS, Gene aiiA encodes the enzyme which degrades AHLs. And gene luxS produces DPD. Therefore, we could realize an AHL-DPD double-cycled oscillation, and the phase difference is half a cycle.Notes
LAA tag has the highest degradation efficiecy in 37℃. Pay attention to the environmental temperature while using it.It can only be used in Gram-negative bacteria.Source
From E coli genome, the subparts of this part is from iGEM Distribution kits.| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public public public public | BBa_R0063 BBa_B0034 BBa_C0062 BBa_B0015 BBa_R0062 BBa_B0034 BBa_C0061 BBa_B0015 BBa_R0063 BBa_B0034 BBa_C0062 BBa_B0015 BBa_R0062 BBa_B0032 BBa_C0060 BBa_B0015 BBa_R0062 BBa_B0034 BBa_K091109 BBa_B0015 | lux pL BBa_B0034 luxr BBa_B0015 lux pR BBa_B0034 luxI BBa_B0015 lux pL BBa_B0034 luxr BBa_B0015 lux pR BBa_B0032 aiiA BBa_B0015 lux pR BBa_B0034 BBa_K091109 BBa_B0015 |