BBa_K395005
BBa_K395005 Version 1 (Component)
plux(rep)-middle

BBa_J100282
BBa_J100282 Version 1 (Component)
rClone Red Version 2 with RBS: Device for GGA Cloning and Testing RBS elements and Riboswitches

BBa_J100297
BBa_J100297 Version 1 (Component)
rClone Red Version 1.5 with RBS 2.0: Device for GGA Cloning and Testing RBS elements and Riboswitche

BBa_J100296
BBa_J100296 Version 1 (Component)
rClone Red Version 2 with RBS 2.0: Device for GGA Cloning and Testing RBS elements and Riboswitches

BBa_K1159309
BBa_K1159309 Version 1 (Component)
19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25]

BBa_K325239
BBa_K325239 Version 1 (Component)
Red Firefly Luciferase and LRE (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_S03638
BBa_S03638 Version 1 (Component)
HixC-pBad_rev-HixC : RBS-TetF

BBa_K395006
BBa_K395006 Version 1 (Component)
plux(rep)-small

BBa_K1179007
BBa_K1179007 Version 1 (Component)
(Acyl-TyA-TCS-rtTA3-His) A membrane/exosome targeting rtTA3 fusion with a TEV clevage site

BBa_S03635
BBa_S03635 Version 1 (Component)
HixC-pBad-HixC-RBS-TetF : HixC-TT-RE

SUPPORT DE
BBa_K1433022 Version 1 (Component)
rT-rKan-rRBS-attB-P-attP-RBS-lambda red-RBS-Chl-T-P-RBS-gp47-tag-T

BBa_I715065
BBa_I715065 Version 1 (Component)
Burnt Pancake Tester with RE and backwards Tet resistance

BBa_J96027
BBa_J96027 Version 1 (Component)
TEV cleavage site

BBa_K1319016
BBa_K1319016 Version 1 (Component)
TEV protease cleavage site

BBa_S03674
BBa_S03674 Version 1 (Component)
(-1,2) HixC-pBad_rev-HixC : RBS-TetF-HixC

BBa_K415200
BBa_K415200 Version 1 (Component)
Strong Constitutive T5 Promoter with cmt Operator (T5-cmt)

BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

BBa_K360115
BBa_K360115 Version 1 (Component)
Mutated red luciferase based on BBa_I712019

BBa_J44008
BBa_J44008 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44006
BBa_J44006 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

BBa_J44007
BBa_J44007 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44005
BBa_J44005 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

BBa_K892000
BBa_K892000 Version 1 (Component)
Red light responsive luciferase

BBa_J119395
BBa_J119395 Version 1 (Component)
tClone Red containing the RS3 Riboswitch

TEV cut si
BBa_K128002 Version 1 (Component)
TEV tobacco etch virus protease cleavage site

BBa_I715069
BBa_I715069 Version 1 (Component)
Test part to see if RE and pLac +hixc can be expressed with GFP

BBa_K892999
BBa_K892999 Version 1 (Component)
red light responsive inverter driving luciferase

BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)

BBa_K2017010
BBa_K2017010 Version 1 (Component)
35s + Ga20ox consense + RSIAT-TEV-Luciferase + Tnos

AraC_TEV-F
BBa_K627010 Version 1 (Component)
Fusion of AraC induction system and TEV protease 3

BBa_K1088058
BBa_K1088058 Version 1 (Component)
10 aa linker with BamHI restriction site and TEV recognition site

BBa_K1638006
BBa_K1638006 Version 1 (Component)
10 aa linker with BamHI restriction site and TEV recognition site

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.