BBa_K1111010
BBa_K1111010 Version 1 (Component)
Streptavidin Alive

BBa_M36105
BBa_M36105 Version 1 (Component)
HY5 Negative Control

BBa_J64036
BBa_J64036 Version 1 (Component)
DH domain constitutively active +atg

BBa_K808027
BBa_K808027 Version 1 (Component)
EstA : inactive fusion protein for bacterial surface expression

mnt (s)
BBa_C0072 Version 1 (Component)
mnt repressor (strong) from Salmonella phage P22 (+LVA)

NpuDnaE(C)
BBa_K1362401 Version 1 (Component)
NpuDnaE C-Intein cloning piece

BBa_J35030
BBa_J35030 Version 1 (Component)
GCN4 bZIP binding motive

TetR-4C
BBa_I12212 Version 1 (Component)
TetR - TetR-4C heterodimer promoter (negative)

RFC105 A
BBa_K1362440 Version 1 (Component)
N-terminal start overhang (T)(A)-GATG=RBS+Start RFC[105] A

Stop
BBa_K1362437 Version 1 (Component)
Tripple A

AsLOV2pep
BBa_K1742000 Version 1 (Component)
Avena sativa LOV2 domain

BBa_M36285
BBa_M36285 Version 1 (Component)
Cop Y : negative transcriptional regulator

BBa_K1992008
BBa_K1992008 Version 1 (Component)
Tar GFP tagged, native RBS expression system (promoter+RBS+coding+terminator)

BBa_K390028
BBa_K390028 Version 1 (Component)
GFP with 5'UTR and native RBS from Synechocystis sp. PCC 6803

BBa_I739105
BBa_I739105 Version 1 (Component)
Double Promoter (LuxR/HSL, positive / cI, negative)

BBa_K1362251
BBa_K1362251 Version 1 (Component)
Intein protease

BBa_K1974003
BBa_K1974003 Version 1 (Component)
Orally Active Insecticidal Peptide (OAIP)

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_K112212
BBa_K112212 Version 1 (Component)
{rbs.int!} The integrase gene with native rbs site and stop codon, BBb format

Intein C-t
BBa_K2075024 Version 1 (Component)
Intein and Intein Linker (C-term)

Intein N-t
BBa_K2075010 Version 1 (Component)
Intein and Intein linker (N-term)

BBa_K902084
BBa_K902084 Version 1 (Component)
Prha-B0034-S7(active)

ECFP
BBa_E0024 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

His6
BBa_K1362431 Version 1 (Component)
N-terminal His6 (C-Intein)

xis
BBa_K112205 Version 1 (Component)
{rbs.xis!} The xis gene with native rbs site and stop codon; assembly standard 21

MaoA
BBa_K1615022 Version 1 (Component)
Monoamine Oxidase A RFC25

OmpA
BBa_K103006 Version 1 (Component)
OmpA outer membrane protein A fused to linker; displays proteins on cell surface

BBa_J107026
BBa_J107026 Version 1 (Component)
3OC6HSL-dependent positive/negative switch

BBa_K598015
BBa_K598015 Version 1 (Component)
T7-promoter+TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+GFP+T7-terminator

BBa_K598016
BBa_K598016 Version 1 (Component)
T7-promoter+TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+GFP+T7-terminator

TetR(B)
BBa_I12219 Version 1 (Component)
Wild-type TetR(B) promoter (negative)

BBa_I739104
BBa_I739104 Version 1 (Component)
Double Promoter (LuxR/HSL, positive / P22 cII, negative)

BBa_K729009
BBa_K729009 Version 1 (Component)
Three fold active biological containment System

BBa_K1197015
BBa_K1197015 Version 1 (Component)
Catalytically active CYP6G1 coding device

BBa_K1172915
BBa_K1172915 Version 1 (Component)
Biosafety-System TetOR alive (TetR)

BBa_K1962006
BBa_K1962006 Version 1 (Component)
Truncated Colicin E9 Lacking Bacteriocin Active Domain

BBa_K1962002
BBa_K1962002 Version 1 (Component)
Truncated Colicin Ia Lacking Bacteriocin Active Domain

BBa_K2008000
BBa_K2008000 Version 1 (Component)
Bowman Birk Protease Inhibitor (BBI) active nonamer

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

BBa_K1362050
BBa_K1362050 Version 1 (Component)
Intein protease - T7RBS-His-SspDnaBC

BBa_K209473
BBa_K209473 Version 1 (Component)
pcDNA3.1(+) with AarI A-D sites in MCS

A
BBa_G0009 Version 1 (Component)
The nucleotide A

mnt (w)
BBa_C0073 Version 1 (Component)
mnt repressor (weak) from Salmonella phage P22 (+LVA)

Intein
BBa_K2075002 Version 1 (Component)
Intein

BBa_K729010
BBa_K729010 Version 1 (Component)
Three fold active biological containment System - Antitoxin/Antiholin

BBa_I742118
BBa_I742118 Version 1 (Component)
CrtZ with native ribosome binding site

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Intein
BBa_K2075025 Version 1 (Component)
Intein N- and C-Term + Linker