BBa_I8034
BBa_I8034 Version 1 (Component)
Booperator aiia test 5: effect of tetR on production of LuxR

PLac-CI-La
BBa_I763018 Version 1 (Component)
GFP, LacI, LacY and CI coding device switched on by IPTG

BBa_K079016
BBa_K079016 Version 1 (Component)
RecA promoter with GFP reporter protein on a medium copy number plasmid

BBa_K2066116
BBa_K2066116 Version 1 (Component)
Synthetic Enhancer Project: 3x TetO Binding Cassette (52s) + sfGFP on UNS

BBa_K2066115
BBa_K2066115 Version 1 (Component)
Synthetic Enhancer Project: 2x TetO Binding Cassette (55as) + sfGFP on UNS

BBa_K165092
BBa_K165092 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Gli1 repressor (mCherryx2 tagged) on pRS303

BBa_K165095
BBa_K165095 Version 1 (Component)
Gli1 bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS303

Plac-LacY-
BBa_I763032 Version 1 (Component)
LacY coding device switched on by IPTG with GFP as a reporter

BBa_K1162014
BBa_K1162014 Version 1 (Component)
protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon

BBa_M1722
BBa_M1722 Version 1 (Component)
Part is based on theory. Consists of pBAD promoter, hepcidin and GFP

BBa_K2066120
BBa_K2066120 Version 1 (Component)
Synthetic Enhancer Project: 3X TetO Binding Cassette(52S) + NRII + sfGFP on UNS

BBa_K2066119
BBa_K2066119 Version 1 (Component)
Synthetic Enhancer Project: 2X TetO Binding Cassette(52S) + NRII + sfGFP on UNS

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette

BBa_K165091
BBa_K165091 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS306

BBa_K142029
BBa_K142029 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette

BBa_K142028
BBa_K142028 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette

BBa_K142025
BBa_K142025 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K142031
BBa_K142031 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K142030
BBa_K142030 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette

BBa_I20292
BBa_I20292 Version 1 (Component)
There is no limit to what a man can do or where he can go if...

BBa_K2066121
BBa_K2066121 Version 1 (Component)
Synthetic Enhancer Project: 2X TetO Binding Cassette(55aS) + NRII + TetR + sfGFP on UNS

BBa_K2066122
BBa_K2066122 Version 1 (Component)
Synthetic Enhancer Project: 3X TetO Binding Cassette(52S) + NRII + TetR + sfGFP on UNS

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K1051356
BBa_K1051356 Version 1 (Component)
K1051301(clb2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

BBa_K542012
BBa_K542012 Version 1 (Component)
Catechol 2,3-dioxygenase with a C-terminal Arg-tag and Double Terminator (no promoter/RBS)

Digitalizer
Digitalizer_collection Version 1 (Collection)
A genetic device to digitalize gene expression into a sharp on/off signal.

BBa_K185033
BBa_K185033 Version 1 (Component)
An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31

BBa_M30002
BBa_M30002 Version 1 (Component)
-- No description --

BBa_M30004
BBa_M30004 Version 1 (Component)
-- No description --

BBa_M30005
BBa_M30005 Version 1 (Component)
-- No description --

BBa_M30008
BBa_M30008 Version 1 (Component)
-- No description --

BBa_M30007
BBa_M30007 Version 1 (Component)
-- No description --

BBa_M30010
BBa_M30010 Version 1 (Component)
-- No description --

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_K209000
BBa_K209000 Version 1 (Component)
cAR1 (Dictyostelium discoideum), no STOP

BBa_K121010
BBa_K121010 Version 1 (Component)
Ptet GFP on pSB6

BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

BBa_J36852
BBa_J36852 Version 1 (Component)
Streptavidin, single-chain dimer (no start codon)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

placIQ RBS
BBa_K193604 Version 1 (Component)
GFP behind a constitutive promoter (placIQ) on pSB4A5

placIQ RBS
BBa_K193601 Version 1 (Component)
Constitutive Promoter (placIQ ) + RBS + melA on Low copy vector(pSB6A1)

BBa_K2087000
BBa_K2087000 Version 1 (Component)
CRISPR/Cas9 sgRNA Targeting Locus 618 on the DD96 Human Gene