BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

BBa_K648008
BBa_K648008 Version 1 (Component)
TEV protease cleaveage site with Standard 25 Prefix/Suffix

BBa_K1159110
BBa_K1159110 Version 1 (Component)
Thermonuclease with N-terminal SV40 NLS and flexible linker with TEV site in RFC[25]

BBa_I13457
BBa_I13457 Version 1 (Component)
Improved insertion site with two RNAse E cut sites

TEV Site
BBa_K2075003 Version 1 (Component)
TEV cleavage site

BBa_K1321014
BBa_K1321014 Version 1 (Component)
CBDCipA with N and C-terminal linker

BBa_K1462502
BBa_K1462502 Version 1 (Component)
PDZ domain + Linker

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

BBa_K1150013
BBa_K1150013 Version 1 (Component)
AGS Linker

LytC-Flex-
BBa_K316043 Version 1 (Component)
LytC - Flexible Linker - TEV Cleavage Site - AIP - His Tag

BBa_K1462503
BBa_K1462503 Version 1 (Component)
SH3 domain + Linker

BBa_K1462501
BBa_K1462501 Version 1 (Component)
GBD domian + Linker

linker
BBa_K365005 Version 1 (Component)
20 aa linker RFC-25 (fusion standard Freiburg)

BBa_K1321348
BBa_K1321348 Version 1 (Component)
sfGFP fused to dCBD with linker in RFC 25

BBa_K1321345
BBa_K1321345 Version 1 (Component)
NiBP fused to dCBD with linker in RFC 25

lox
BBa_J61046 Version 1 (Component)
[Lox] site for recombination

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

BBa_K1159102
BBa_K1159102 Version 1 (Component)
C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

TEV clv.
BBa_K1362453 Version 1 (Component)
TEV protease recognition/cleavage site

Linker
BBa_K1088051 Version 1 (Component)
10 aa linker with BamHI restriction site and TEV recognition site

BBa_K1159201
BBa_K1159201 Version 1 (Component)
Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_K1159318
BBa_K1159318 Version 1 (Component)
TEV cleavage site flanked by Gly for better accessibility in RFC[25]

BBa_K1321009
BBa_K1321009 Version 1 (Component)
Nickel Binding Protein (NiBP) Freiburg format (RFC[25])

BBa_K249006
BBa_K249006 Version 1 (Component)
Fusion EYFP with C-terminal Arginine Tag

BBa_K323107
BBa_K323107 Version 1 (Component)
PBSII zinc finger + linker

BBa_K1159101
BBa_K1159101 Version 1 (Component)
N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

BBa_K1321351
BBa_K1321351 Version 1 (Component)
CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)

BBa_K1486003
BBa_K1486003 Version 1 (Component)
Flexible linker 2x (GGGS)

RFC105 NC
BBa_K1362417 Version 1 (Component)
C-terminal GG-linker overhang GG<u>TGGT</u>=Gly+Gly RFC[105] overhang NC

linker
BBa_K157009 Version 1 (Component)
Split fluorophore linker; Freiburg standard

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

LytC-Heli-
BBa_K316044 Version 1 (Component)
LytC - Helical Linker - TEV Cleavage Site - AIP - His Tag

LytC-Gly-T
BBa_K316042 Version 1 (Component)
LytC - Glycine Linker - TEV Cleavage Site - AIP - His Tag

BsaI ->
BBa_K1362425 Version 1 (Component)
BsaI restriction site for RFC[???] cloning (in part in prefix)

BBa_I13452
BBa_I13452 Version 1 (Component)
Adds another BioBrick site

BBa_K2017006
BBa_K2017006 Version 1 (Component)
RSIAT-TEV linker + Luciferase

P-RBS-CWB-
BBa_K316033 Version 1 (Component)
Pveg-spoVG-LytC-Flexible Linker-TEV Cleavage Site-AIP-His Tag

BBa_I712016
BBa_I712016 Version 1 (Component)
myristoilation signal sequence fused to Tobacco Etch Virus (TEV) protease cleavage site

TEV clv.
BBa_K1362452 Version 1 (Component)
TEV protease recognition/cleavage site

<- BsaI
BBa_K1362423 Version 1 (Component)
BsaI reverse restriction site for RFC[105] cloning

BBa_K648009
BBa_K648009 Version 1 (Component)
RecA Cleavage Site with Standard 25 Prefix/Suffix

BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

BBa_K1321011
BBa_K1321011 Version 1 (Component)
SmtA metallothionein in Freiburg format (RFC[25])

LytC-Flex-
BBa_K316046 Version 1 (Component)
LytC - Flexible Linker - Elastase Cleavage Site - AIP - His Tag