Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

GFP
GFP Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S

HYG
Hygromicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

NEO (G418/Neomicin Resistance)
Neomicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

mCherry
mCherry Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

amdS
amdS Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

APP1
GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

ACT1
GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_K1937008
BBa_K1937008 Version 1 (Component)
AF-A antifungal operon in pSBBs0K-mini

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

cinI
BBa_C0076 Version 1 (Component)
autoinducer synthetase

BBa_K1937007
BBa_K1937007 Version 1 (Component)
AF-A : operon of antifungal peptides

BBa_K1937001
BBa_K1937001 Version 1 (Component)
pSBBsOK-mini, a small replicative plasmid for Bacillus subtilis

BBa_I13458
BBa_I13458 Version 1 (Component)
promoter C and arabinose C operon

BBa_I746104
BBa_I746104 Version 1 (Component)
P2 promoter in agr operon from S. aureus

BBa_K1937006
BBa_K1937006 Version 1 (Component)
pNagP-RFP in pSBBs0K-mini plasmid

BBa_K1937004
BBa_K1937004 Version 1 (Component)
pNagA-RFP in pSBBs0K-mini plasmid

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_J54103
BBa_J54103 Version 1 (Component)
Promoterless-GFP(A)

BBa_K1937010
BBa_K1937010 Version 1 (Component)
Epsilon/MazF toxin and antitoxin in pSBBS0K-mini

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K209402
BBa_K209402 Version 1 (Component)
AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

GAFP-1
BBa_K1364002 Version 1 (Component)
RBS - Antifungal GAFP-1

GAFP-1
BBa_K1364007 Version 1 (Component)
RBS + Antifungal GAFP-1 + Double terminator

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

lacZ a
BBa_E0033 Version 1 (Component)
LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)

phaC1-A-B1
BBa_K934001 Version 1 (Component)
phaC1-A-B1 [P(3HB) synthesis]

BBa_K322123
BBa_K322123 Version 1 (Component)
Phycobilin synthesis operon without terminator

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission