Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

BBa_K1362050
BBa_K1362050 Version 1 (Component)
Intein protease - T7RBS-His-SspDnaBC

BBa_K1362051
BBa_K1362051 Version 1 (Component)
Intein protease with arabinose inducible regulatory promoter/ repressor araC-Pbad-T7RBS-His-SspDnaBC

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_I712015
BBa_I712015 Version 1 (Component)
HIV-1 protease cleavage site

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_K1362251
BBa_K1362251 Version 1 (Component)
Intein protease

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

BBa_K1362466
BBa_K1362466 Version 1 (Component)
Intein protease extein

SspDnaB(N)
BBa_K1362140 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] assembly construct (with His6)

SspDnaB(C)
BBa_K1362141 Version 1 (Component)
RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

Intein
BBa_K300004 Version 1 (Component)
Engineered pH-inducible intein (codon optimized for E. coli) - internal domain

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

SspDnaB(N)
BBa_K1362408 Version 1 (Component)
SspDnaB N-Intein cloning piece

SspDnaX(N)
BBa_K1362110 Version 1 (Component)
RBS + SspDnaX N-intein RFC[105] assembly construct (with His6)

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_K1362111
BBa_K1362111 Version 1 (Component)
RBS + SspDnaX C-intein RFC[105] assembly construct (with His6)

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

Hin
BBa_J31000 Version 1 (Component)
DNA-invertase Hin from Salmonella typhimurium

His
BBa_K157011 Version 1 (Component)
His affinity tag; Freiburg standard

His6
BBa_K1362431 Version 1 (Component)
N-terminal His6 (C-Intein)

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

SspDnaX(N)
BBa_K1362402 Version 1 (Component)
SspDnaX N-Intein cloning piece

SspDnaX(C)
BBa_K1362403 Version 1 (Component)
SspDnaX C-Intein cloning piece

SspDnaE(N)
BBa_K1362410 Version 1 (Component)
SspDnaE N-Intein cloning piece

BBa_B0053
BBa_B0053 Version 1 (Component)
Terminator (His)

SspDnaE(C)
BBa_K1362411 Version 1 (Component)
SspDnaE C-Intein cloning piece

NpuDnaE(N)
BBa_K1362400 Version 1 (Component)
NpuDnaE N-Intein cloning piece

Intein
BBa_K1483003 Version 1 (Component)
Ssp GyrB Split Intein

Intein
BBa_K1947002 Version 1 (Component)
Intein

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

His-Scaffo
BBa_K1602027 Version 1 (Component)
Protein scaffold with N-terminal HisTag

NpuDnaE(C)
BBa_K1362401 Version 1 (Component)
NpuDnaE C-Intein cloning piece

Intron
BBa_J176014 Version 1 (Component)
Intron

HIV-1
BBa_I712667 Version 1 (Component)
HIV-1 aspartyl protease

Intein C-t
BBa_K2075024 Version 1 (Component)
Intein and Intein Linker (C-term)

Intein N-t
BBa_K2075010 Version 1 (Component)
Intein and Intein linker (N-term)

SspDnaE(N)
BBa_K1362150 Version 1 (Component)
RBS + SspDnaE N-intein RFC[105] assembly construct (with His6)

SspDnaE(C)
BBa_K1362151 Version 1 (Component)
RBS + SspDnaE C-intein RFC[105] assembly construct (with His6)

BBa_K200028
BBa_K200028 Version 1 (Component)
Protease resistant PAH

SspDnaB(C*
BBa_K1362143 Version 1 (Component)
RBS + SspDnaB C-intein RFC[105] non-splicing control construct (with His6)

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

eGFP+His
BBa_K193202 Version 1 (Component)
EGFP(enhanced Green Fluorescent Protein) with His tag