Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K1159002
BBa_K1159002 Version 1 (Component)
Laccase from <i>Bacillus pumilus</i> in RFC[25]

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_K863001
BBa_K863001 Version 1 (Component)
bpul laccase from Bacillus pumilus

RFC 37
BBa_K245009 Version 1 (Component)
RFC 37 Scar Sequence

BBa_K1321005
BBa_K1321005 Version 1 (Component)
Synthetic Phytochelatin EC(20) RFC[25]

BBa_K1321337
BBa_K1321337 Version 1 (Component)
sfGFP in Freiburg format (RFC 25)

BBa_K1159303
BBa_K1159303 Version 1 (Component)
Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25]

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K1321339
BBa_K1321339 Version 1 (Component)
CBDcenA+Linker, RFC 25 standard

penI
BBa_C0074 Version 1 (Component)
penI repressor from Bacillus licheniformis (+LVA)

BBa_K863000
BBa_K863000 Version 1 (Component)
bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

BBa_K1321009
BBa_K1321009 Version 1 (Component)
Nickel Binding Protein (NiBP) Freiburg format (RFC[25])

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K1159304
BBa_K1159304 Version 1 (Component)
Signal Peptide of Ig Kappa chain from <i>Mus musculus</i> in RFC[25] N-part

phlF
BBa_K1725040 Version 1 (Component)
<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5

p_qrr4
BBa_K131017 Version 1 (Component)
p_qrr4 from <I>Vibrio harveyi</I>

BBa_K733001
BBa_K733001 Version 1 (Component)
<i>Ptms</i> - a constitutive promoter in <i>E. coli</i> and <i>B. subtilis</i>.

BBa_K863021
BBa_K863021 Version 1 (Component)
bhal laccase from Bacillus halodurans

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_K823001
BBa_K823001 Version 1 (Component)
P_<i>liaI</i> promoter of <i>Bacillus subtilis</i>

BBa_K1321011
BBa_K1321011 Version 1 (Component)
SmtA metallothionein in Freiburg format (RFC[25])