Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

SEGA021
SEGA021 Version 1 (Component)
natural linear DNA.

SEGA022
SEGA022 Version 1 (Component)
natural linear DNA.

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

LytC-Flex-
BBa_K316046 Version 1 (Component)
LytC - Flexible Linker - Elastase Cleavage Site - AIP - His Tag

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

LytC-Gly-E
BBa_K316045 Version 1 (Component)
LytC - Glycine Linker - Elastase Cleavage Site - AIP - His Tag

LytC-Heli-
BBa_K316047 Version 1 (Component)
LytC - Helical Linker - Elastase Cleavage Site - AIP - His Tag

LytC-Flex-
BBa_K316043 Version 1 (Component)
LytC - Flexible Linker - TEV Cleavage Site - AIP - His Tag

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

BBa_I712015
BBa_I712015 Version 1 (Component)
HIV-1 protease cleavage site

P-RBS-CWB-
BBa_K316036 Version 1 (Component)
Pveg-spoVG-LytC-Flexible Linker-Elastase Cleavage Site-AIP-His Tag

BBa_J61100
BBa_J61100 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

BBa_J85600
BBa_J85600 Version 1 (Component)
attB1 recombination site

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

LytC-Heli-
BBa_K316044 Version 1 (Component)
LytC - Helical Linker - TEV Cleavage Site - AIP - His Tag

LytC-Gly-T
BBa_K316042 Version 1 (Component)
LytC - Glycine Linker - TEV Cleavage Site - AIP - His Tag

P-RBS-CWB-
BBa_K316037 Version 1 (Component)
Pveg-spoVG-LytC-Helical Linker-Elastase Cleavage Site-AIP-His Tag

P-RBS-CWB-
BBa_K316035 Version 1 (Component)
Pveg-spoVG-LytC-Glycine Linker-Elastase Cleavage Site-AIP-His Tag

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

P-RBS-CWB-
BBa_K316033 Version 1 (Component)
Pveg-spoVG-LytC-Flexible Linker-TEV Cleavage Site-AIP-His Tag

BBa_K1486004
BBa_K1486004 Version 1 (Component)
flexible linker 2x (GGGGS)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K1123012
BBa_K1123012 Version 1 (Component)
His-Tag and Thrombine Cleavage Site

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

P-RBS-CWB-
BBa_K316034 Version 1 (Component)
Pveg-spoVG-LytC-Helical Linker-TEV Cleavage Site-AIP-His Tag

P-RBS-CWB-
BBa_K316032 Version 1 (Component)
Pveg-spoVG-LytC-Glycine Linker-TEV Cleavage Site-AIP-His Tag

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

NLS
BBa_J176008 Version 1 (Component)
NLS

PEST
BBa_J176018 Version 1 (Component)
PEST