Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

mCh
BBa_J176005 Version 1 (Component)
mCh

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

fshPCD
BBa_J176002 Version 1 (Component)
fshPCD

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

VP64
BBa_J176013 Version 1 (Component)
VP64

PLflex
BBa_J176040 Version 1 (Component)
PLflex

BBa_J176130
BBa_J176130 Version 1 (Component)
PLflex4

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_J176132
BBa_J176132 Version 1 (Component)
PLrigid4

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_K1462503
BBa_K1462503 Version 1 (Component)
SH3 domain + Linker

LuxR
BBa_I0462 Version 1 (Component)
luxR Protein Generator

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

BBa_K165006
BBa_K165006 Version 1 (Component)
Zif268-HIV DNA-binding domain

BBa_K300002
BBa_K300002 Version 1 (Component)
Phasin (PhaP) - head domain

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

Fok_a
BBa_K243000 Version 1 (Component)
Protein domain (active) of the restriction endonuclease FokI

RFC105 CC
BBa_K1362419 Version 1 (Component)
C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC

Gal4DB
BBa_J176020 Version 1 (Component)
Gal4 DB

BBa_J70594
BBa_J70594 Version 1 (Component)
RFC12 TAATAA Tail Domain

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_K1462502
BBa_K1462502 Version 1 (Component)
PDZ domain + Linker

SPzim17
BBa_K809201 Version 1 (Component)
signal peptide of ZIM17

mCherry
BBa_K165004 Version 1 (Component)
mCherry, yeast optimized for fusion

BBa_K1321002
BBa_K1321002 Version 1 (Component)
Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25

BBa_K1321003
BBa_K1321003 Version 1 (Component)
Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

NpuDnaE(N)
BBa_K1362400 Version 1 (Component)
NpuDnaE N-Intein cloning piece