Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_J36843
BBa_J36843 Version 1 (Component)
Streptavidin, single-chain dimer + His6 tag (no start codon)

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

BBa_K809601
BBa_K809601 Version 1 (Component)
GAL promoter (kozak) without start codon ATG

BBa_J36841
BBa_J36841 Version 1 (Component)
Streptavidin, wild-type core + His6 tag (no start codon)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_J36842
BBa_J36842 Version 1 (Component)
Streptavidin, dead (no start codon)

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K1583059
BBa_K1583059 Version 1 (Component)
His-tag without start codon

BBa_K2066518
BBa_K2066518 Version 1 (Component)
mCherry (with start codon)

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

BBa_M36009
BBa_M36009 Version 1 (Component)
5' Bicistronic UTR (strong), does not include ATG start

PEST
BBa_J176018 Version 1 (Component)
PEST

T7rnap
BBa_I716103 Version 1 (Component)
T7rnap (T7 RNA Polymerase) - ATG Start Codon

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K1333002
BBa_K1333002 Version 1 (Component)
M13 bacteriophage g3p with start codon of "ATG" and a FLAG tag on C-terminal

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_J36840
BBa_J36840 Version 1 (Component)
Streptavidin, wild-type core (no start codon)

rhIR
BBa_C0171 Version 1 (Component)
rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

BBa_K641006
BBa_K641006 Version 1 (Component)
LambdaRep (no start codon)

BBa_J36852
BBa_J36852 Version 1 (Component)
Streptavidin, single-chain dimer (no start codon)

BBa_J36847
BBa_J36847 Version 1 (Component)
Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6

BBa_J36845
BBa_J36845 Version 1 (Component)
Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

BBa_K215001
BBa_K215001 Version 1 (Component)
Secretion Signal and Streptavidin Binding Tags

BBa_M36282
BBa_M36282 Version 1 (Component)
5' Bicistronic UTR, includes ATG start codon

BBa_I716210
BBa_I716210 Version 1 (Component)
BamHI endonuclease without start codon

BBa_K2144009
BBa_K2144009 Version 1 (Component)
Sequence for His6-tag and LPXTGG-tag

BBa_J36849
BBa_J36849 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag

BBa_J36851
BBa_J36851 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag

BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

BBa_K1223006
BBa_K1223006 Version 1 (Component)
His-Tag

BBa_K323217
BBa_K323217 Version 1 (Component)
T7 promoter with start codon and hexahistidine tag

aiiA
BBa_C0160 Version 1 (Component)
autoinducer inactivation enzyme aiiA (no LVA)

T7start
BBa_J18912 Version 1 (Component)
T7 promoter + RBS + START