URA5
GenBank_CM000046_Region_302227_303005 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

URA5
GenBank_CM000046_Region_301512_302226 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

URA5
GenBank_CM000046_Region_303006_303540 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

amilCP
BBa_K592009 Version 1 (Component)
amilCP, blue chromoprotein

BBa_K2180005
BBa_K2180005 Version 1 (Component)
Pigment Blue for B. subtilis with a LVA tail and restriction site (Blue Pigment)

BBa_K2180020
BBa_K2180020 Version 1 (Component)
Blue pigment adaptated with B. subtilis code codon with LVA tail

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

BBa_J64101
BBa_J64101 Version 1 (Component)
ColE1 biobrick plasmid with backward terminator

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

GFP
BBa_K145015 Version 1 (Component)
GFP with LVA tag

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

Pveg-spoVG
BBa_K143053 Version 1 (Component)
Promoter Pveg and RBS spoVG for B. subtilis

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

BBa_J09855
BBa_J09855 Version 1 (Component)
Constitutive luxR device with pLuxR

BBa_K228000
BBa_K228000 Version 1 (Component)
T7ptag(T7polymerase with amber mutation)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

Pveg
BBa_K143012 Version 1 (Component)
Promoter veg a constitutive promoter for B. subtilis

BBa_K2066527
BBa_K2066527 Version 1 (Component)
B0034 with scar TACTAG

eyfp
BBa_E0031 Version 1 (Component)
EYFP (RBS+ LVA-)

BFP
BBa_K592100 Version 1 (Component)
Blue Fluorescent Protein (mTagBFP)

tetA(C)b
BBa_J31006 Version 1 (Component)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

BBa_K1051006
BBa_K1051006 Version 1 (Component)
Saccharomycescerevisiae TBY-1 terminator with stopcodon

BBa_K592016
BBa_K592016 Version 1 (Component)
B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_K2180019
BBa_K2180019 Version 1 (Component)
Blue Pigment for B. subtilis

ecfp
BBa_E0021 Version 1 (Component)
ECFP (RBS+ LVA-)

BBa_K209402
BBa_K209402 Version 1 (Component)
AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

BLp
BBa_K238013 Version 1 (Component)
Exact promoter of the blue light receptor

BBa_K082003
BBa_K082003 Version 1 (Component)
GFP(+LVA)

cI HK022
BBa_C0050 Version 1 (Component)
cI repressor from phage HK022 (+LVA?)

BBa_K773003
BBa_K773003 Version 1 (Component)
mCherry-LVA