BBa_K1916013
BBa_K1916013 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1916007
BBa_K1916007 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1916014
BBa_K1916014 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

c-Myc
BBa_M0032 Version 1 (Component)
c-Myc affinity tag

BBa_K1159995
BBa_K1159995 Version 1 (Component)
10/25 N-Part Assembly Scar

BBa_K331018
BBa_K331018 Version 1 (Component)
Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with N-Terminal Arginine Tag)

com C
BBa_K233302 Version 1 (Component)
competence factor C , Acinetobacter BD413

BBa_K1446001
BBa_K1446001 Version 1 (Component)
dcmA-gene with N-terminal pdu-microcompartment tag, codon optimised for E.coli (K12)

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

BBa_K1833009
BBa_K1833009 Version 1 (Component)
T7 promoter -> 434 cI N terminus with P22 repressor specificity

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1022122
BBa_K1022122 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II : RBS TetR :TT

eGFP+His
BBa_K193202 Version 1 (Component)
EGFP(enhanced Green Fluorescent Protein) with His tag

BBa_K1128009
BBa_K1128009 Version 1 (Component)
Methyl CpG Binding Protein 2 with N-Terminus Cut off

BBa_K738005
BBa_K738005 Version 1 (Component)
FB-2X-PP7: Split GFP C-terminal domain fused with PP7 protein

H6-sfGFP(N
BBa_K1362471 Version 1 (Component)
split sfGFPn half

BBa_K1362004
BBa_K1362004 Version 1 (Component)
Sortase A non-circularization construct (with His6)

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K209408
BBa_K209408 Version 1 (Component)
AarI C-D part, Intersectin (ITSN)

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_K209411
BBa_K209411 Version 1 (Component)
AarI C-D part, Lamellipodin (LPD) (775-1250)

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1791003
BBa_K1791003 Version 1 (Component)
6X end terminally His tagged MS2 coat protein

BBa_K209015
BBa_K209015 Version 1 (Component)
Aar1 C-D part, Inp54p

BBa_K331019
BBa_K331019 Version 1 (Component)
Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with C-Terminal Arginine Tag)

BBa_K2170132
BBa_K2170132 Version 1 (Component)
C-terminal intermediate of eukaryotic hypoxie sensor (VEGF-p2A-PDGF_polyA) in RFC[10]

BBa_K209409
BBa_K209409 Version 1 (Component)
AarI C-D part, Tuba

BBa_K1159110
BBa_K1159110 Version 1 (Component)
Thermonuclease with N-terminal SV40 NLS and flexible linker with TEV site in RFC[25]

BBa_K1362300
BBa_K1362300 Version 1 (Component)
NpuDnaE N-Intein RFC[105] assembly construct (with His6)

BBa_K929200
BBa_K929200 Version 1 (Component)
N-terminal sortase motif linked to AAV2-VP2 with Myc-tag, kozak and CMV

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

BBa_K1362202
BBa_K1362202 Version 1 (Component)
Sortase A circularization construct (with His6; without RBS)

BBa_K1128008
BBa_K1128008 Version 1 (Component)
Methyl CpG Binding Protein 2 with C-Terminus Cut off

BBa_K209001
BBa_K209001 Version 1 (Component)
Aar1 C-D, PTEN (Dictyostelium discoideum), no STOP

BBa_K115020
BBa_K115020 Version 1 (Component)
RNA thermometer (ROSE hairpin 37??C)

BBa_K1362204
BBa_K1362204 Version 1 (Component)
Sortase A non-circularization construct (with His6; without RBS)

BBa_K1997000
BBa_K1997000 Version 1 (Component)
sHRP-N

C-IgAP
BBa_J32018 Version 1 (Component)
C-IgAP

BBa_K392014
BBa_K392014 Version 1 (Component)
C. josui CM10