BBa_M50020
BBa_M50020 Version 1 (Component)
Promoter for GolS + GFP

BBa_J09266
BBa_J09266 Version 1 (Component)
constitutive GFP (AAV+) device, medium RBS

BBa_K863201
BBa_K863201 Version 1 (Component)
3' UTR site of alcohol oxidase 1 gene (aox1)

BBa_K313013
BBa_K313013 Version 1 (Component)
loading sequence of RNA phage MS2 (reverse)

BBa_I2011
BBa_I2011 Version 1 (Component)
B_Receiver Device (R1075, B0034.C0071.B0015)

BBa_K1614002
BBa_K1614002 Version 1 (Component)
transcription cassette of BBF RFC 110

xynA-PDZli
BBa_K1602035 Version 1 (Component)
Inducible generator of RBS-xynA-PDZlig

BBa_K2139001
BBa_K2139001 Version 1 (Component)
Coding Sequence of Endo5a

BBa_K1633005
BBa_K1633005 Version 1 (Component)
MOR siRNA-3 (siRNA for mouse Mu opioid receptor)

BBa_K1633004
BBa_K1633004 Version 1 (Component)
MOR siRNA-2 (siRNA for mouse Mu opioid receptor)

BBa_K1633003
BBa_K1633003 Version 1 (Component)
MOR siRNA-1 (siRNA for mouse Mu opioid receptor)

BBa_K1633006
BBa_K1633006 Version 1 (Component)
MOR siRNA-4 (siRNA for mouse Mu opioid receptor)

BBa_K1149006
BBa_K1149006 Version 1 (Component)
pelB-pulA-flag expression

BBa_K1647018
BBa_K1647018 Version 1 (Component)
CAP-Bac8c, an improved antimicrobial peptide selectively

test
BBa_K1479015 Version 1 (Component)
Just for test

BBa_M45905
BBa_M45905 Version 1 (Component)
Composite part for fluorescent detection of botulinum neurotoxin A (BoNT A)

Excel2SBOL
Excel2SBOL_collection Version 1 (Collection)
A collection of sbol that is used for the excel2sbol library

SPtom22
BBa_K809206 Version 1 (Component)
signal peptide of Tom22

BBa_T4002
BBa_T4002 Version 1 (Component)
luxC device regulated by lambda cl

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1351000
BBa_K1351000 Version 1 (Component)
beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC

BBa_M11005
BBa_M11005 Version 1 (Component)
comE3 Competence locus E from H. pylori

BBa_K1916112
BBa_K1916112 Version 1 (Component)
Ara-Inducible CBCR3 Expression System

BBa_K1916115
BBa_K1916115 Version 1 (Component)
Ara-Inducible CBCR4 Expression System

BBa_K1916101
BBa_K1916101 Version 1 (Component)
Ara-Inducible NpF2164g5 Expression System

BBa_K1916117
BBa_K1916117 Version 1 (Component)
Ara-Inducible CBCR6 Expression System

BBa_K1916121
BBa_K1916121 Version 1 (Component)
Ara-Inducible CBCR9 Expression System

BBa_K2129004
BBa_K2129004 Version 1 (Component)
EutS with Mid expression cassette

BBa_K1916118
BBa_K1916118 Version 1 (Component)
Ara-Inducible CBCR8 Expression System

BBa_K1916120
BBa_K1916120 Version 1 (Component)
Ara-Inducible CBCR9 Expression System

BBa_K1916122
BBa_K1916122 Version 1 (Component)
Ara-Inducible CBCR11 Expression System

BBa_K1916104
BBa_K1916104 Version 1 (Component)
Ara-Inducible Ga0100955_11928g3 Expression System

BBa_J72192
BBa_J72192 Version 1 (Component)
manB and neuS expression cassette

BBa_K1916119
BBa_K1916119 Version 1 (Component)
Ara-Inducible CBCR8 Expression System

BBa_K1916123
BBa_K1916123 Version 1 (Component)
Ara-Inducible CBCR11 Expression System

BBa_K1916107
BBa_K1916107 Version 1 (Component)
Ara-Inducible Ga0039498_104087g2 Expression System

BBa_K1916105
BBa_K1916105 Version 1 (Component)
Ara-Inducible Ga0100955_11928g3 Expression System

BBa_K1916103
BBa_K1916103 Version 1 (Component)
Ara-Inducible Cha6605_4344g2 Expression System

BBa_K1916108
BBa_K1916108 Version 1 (Component)
Ara-Inducible Osc7112_5903g3 Expression System

BBa_K1916116
BBa_K1916116 Version 1 (Component)
Ara-Inducible CBCR6 Expression System

BBa_K2129005
BBa_K2129005 Version 1 (Component)
EutS with Low expression cassette

BBa_K1916114
BBa_K1916114 Version 1 (Component)
Ara-Inducible CBCR4 Expression System

BBa_K1916100
BBa_K1916100 Version 1 (Component)
Ara-Inducible NpF2164g5 Expression System

BBa_K1916113
BBa_K1916113 Version 1 (Component)
Ara-Inducible CBCR3 Expression System

BBa_K1916106
BBa_K1916106 Version 1 (Component)
Ara-Inducible Ga0039498_104087g2 Expression System

BBa_K1916109
BBa_K1916109 Version 1 (Component)
Ara-Inducible Osc7112_5903g3 Expression System

BBa_K1916102
BBa_K1916102 Version 1 (Component)
Ara-Inducible Cyan7427_1390g3 Expression System

BBa_K1916110
BBa_K1916110 Version 1 (Component)
Ara-Inducible CBCR2 Expression System

BBa_K1111005
BBa_K1111005 Version 1 (Component)
Constitutive promoter inducing GFP expression

BBa_K1916111
BBa_K1916111 Version 1 (Component)
Ara-Inducible CBCR2 Expression System