BBa_K136070
BBa_K136070 Version 1 (Component)
flhB Promoter (+ WT RBS) - EnvZ* - GFP generator

BBa_K2150020
BBa_K2150020 Version 1 (Component)
pT7+pTet+RBS+GFP+RBS+T7RNAP+DT

BBa_K136069
BBa_K136069 Version 1 (Component)
fliL promoter (+WT RBS) - EnvZ*- GFP generator

BBa_I737004
BBa_I737004 Version 1 (Component)
AraC, arabinose activated, self sustaining GFP reporter.

BBa_K1649003
BBa_K1649003 Version 1 (Component)
YF1 blue light sensor with GFP reporter

BBa_K1014011
BBa_K1014011 Version 1 (Component)
RFP Protein Generator(S)

BBa_K2036027
BBa_K2036027 Version 1 (Component)
pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag

BBa_T9001
BBa_T9001 Version 1 (Component)
GFP and Producer Controlled by 3OC6HSL Receiver Device

BBa_K175024
BBa_K175024 Version 1 (Component)
PLacI+Key3c+TT+PLambdain+RBS+GFP+TT

BBa_K175026
BBa_K175026 Version 1 (Component)
PLacI+Key3c+TT+PTet+Lock3c+GFP+TT

BBa_I766337
BBa_I766337 Version 1 (Component)
FYVE-EEA1-GFP under medium constitutive promoter

BBa_K604000
BBa_K604000 Version 1 (Component)
autoinducer synthetase rhlI and GFP under PyeaR

BBa_K223060
BBa_K223060 Version 1 (Component)
5MT Inverter-GFP Reporter and Lac Promoter

BBa_K133104
BBa_K133104 Version 1 (Component)
CMV-SS-HA-(TMTIR)TLR4-GFP-Histop

BBa_K392038
BBa_K392038 Version 1 (Component)
GFP surface display testing construct for yeast

BBa_K223047
BBa_K223047 Version 1 (Component)
SoxR - SoxS - GFP Reporter with Lac Promoter

BBa_J22211
BBa_J22211 Version 1 (Component)
rbs LuxR rbs YFP term

BBa_K678007
BBa_K678007 Version 1 (Component)
eGFP+K GOI

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J58103
BBa_J58103 Version 1 (Component)
Mutated promoter activated by OmpR-P with the reporter GFP

BBa_K338062
BBa_K338062 Version 1 (Component)
GFP Reporter Device, Heat-Shock or IPTG Induced

BBa_K199054
BBa_K199054 Version 1 (Component)
RFPrev-RBSrev-pOmpC-pLuxrev-RBS-GFP-TT

BBa_K1334026
BBa_K1334026 Version 1 (Component)
IPTG inducible E7 protein and GFP generator

BBa_K091217
BBa_K091217 Version 1 (Component)
LacI I12 X86 and pLac plus GFP

BBa_K258011
BBa_K258011 Version 1 (Component)
Oxygen-dependent EGF synthesizing with GFP reporter

BBa_J692101
BBa_J692101 Version 1 (Component)
CviR-vioA Receiver v2.00 Characterisation Device (GFP)

BBa_K091218
BBa_K091218 Version 1 (Component)
LacI I12 X86 and pLacIQ plus GFP

BBa_K122006
BBa_K122006 Version 1 (Component)
Amber Suppressed RFP

BBa_K1682005
BBa_K1682005 Version 1 (Component)
<i>P_kdpF</i> - GFP generator

BBa_K2039004
BBa_K2039004 Version 1 (Component)
Coding sequences of proteins FRB* and GFP11 (subunit of tripartite GFP)

BBa_K2039003
BBa_K2039003 Version 1 (Component)
Coding sequences of proteins FKBP and GFP10 (subunit of tripartite GFP)

BBa_K390057
BBa_K390057 Version 1 (Component)
pilA1 promoter, 5'UTR, and RBS with GFP

BBa_K528014
BBa_K528014 Version 1 (Component)
RBS measurement device J23100 B0034 Super Folder GFP

BBa_K1890021
BBa_K1890021 Version 1 (Component)
GFP with strong constitutive promoter, RBS and terminator

BBa_K390058
BBa_K390058 Version 1 (Component)
pixJI promoter, 5'UTR, and RBS with GFP

BBa_K390050
BBa_K390050 Version 1 (Component)
psbA2 promoter, 5'UTR, and RBS with GFP

BBa_K390060
BBa_K390060 Version 1 (Component)
sll0837 promoter, 5'UTR, and RBS with GFP

BBa_K528012
BBa_K528012 Version 1 (Component)
RBS measurement device J23100 B0032 Super Folder GFP

BBa_K1890020
BBa_K1890020 Version 1 (Component)
GFP with strong constitutive promoter, RBS and terminator

BBa_K528013
BBa_K528013 Version 1 (Component)
RBS measurement device J23100 B0033 Super Folder GFP

BBa_K1890024
BBa_K1890024 Version 1 (Component)
GFP with weak constitutive promoter, RBS and terminator

BBa_K390047
BBa_K390047 Version 1 (Component)
hspA promoter, 5'UTR, and RBS with GFP

BBa_I2035
BBa_I2035 Version 1 (Component)
T7 RNAP dependent, lacI regulated GFP expression system

BBa_K390052
BBa_K390052 Version 1 (Component)
glnBP2 promoter, 5'UTR, and RBS with GFP

BBa_K1890023
BBa_K1890023 Version 1 (Component)
GFP with weak constitutive promoter, RBS and terminator

BBa_K390059
BBa_K390059 Version 1 (Component)
sigF promoter, 5'UTR, and RBS with GFP

placIQ RBS
BBa_K193603 Version 1 (Component)
GFP behind a constitutive promoter (placIQ) on pSB6A1

BBa_K414006
BBa_K414006 Version 1 (Component)
35S Promoter + (Strong Plant RBS + GFP) From K414001

BBa_K390056
BBa_K390056 Version 1 (Component)
sigE promoter, 5'UTR, and RBS with GFP

BBa_K390054
BBa_K390054 Version 1 (Component)
psbD2 promoter, 5'UTR, and RBS with GFP