BBa_K1172913
BBa_K1172913 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO Barnase)

BBa_K1351040
BBa_K1351040 Version 1 (Component)
pBS0K<i>Pspac</i>, an IPTG-inducible replicative expression vector for

BBa_J72117
BBa_J72117 Version 1 (Component)
BBb High copy entry vector, pBca1256

BBa_K1172912
BBa_K1172912 Version 1 (Component)
Part 1 of the Biosystem TetOR alive (pRha TetR alr)

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_I732091
BBa_I732091 Version 1 (Component)
Double Repoters (LacZ-alpha and GFP-AAV)

BBa_K1172914
BBa_K1172914 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO GFP)

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

BBa_M36745
BBa_M36745 Version 1 (Component)
The sensor codes for AraC, a transcription factor, which represses the operon.Arabinose induces tran

BBa_K106693
BBa_K106693 Version 1 (Component)
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

BBa_M36475
BBa_M36475 Version 1 (Component)
Codes for AraC, a transcription factor which represses operon. Arabinose dislodges TF protein.

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;24 followed by YFP

BBa_K086008
BBa_K086008 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;28 followed by YFP

BBa_K086006
BBa_K086006 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;28 followed by YFP

BBa_K086005
BBa_K086005 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;28 followed by YFP

BBa_K086009
BBa_K086009 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;32 followed by YFP

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;24 followed by YFP reporter

BBa_K086012
BBa_K086012 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;32 followed by YFP reporter

BBa_K086007
BBa_K086007 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;28 followed by YFP reporter

BBa_K086013
BBa_K086013 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;38 followed by YFP reporter

BBa_K086004
BBa_K086004 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;24 followed by YFP promoter

BBa_K086014
BBa_K086014 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;38 followed by YFP reporter

BBa_K086016
BBa_K086016 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;38 followed by YFP reporter

BBa_K086011
BBa_K086011 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;32 followed by YFP reporter

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;24 followed by YFP reporter

BBa_K086010
BBa_K086010 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;32 followed by YFP reporter

BBa_K086015
BBa_K086015 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor &#963;38 followed by YFP reporter

BBa_K1039021
BBa_K1039021 Version 1 (Component)
Bxb1 integrase and Recombination Directionality Factor (RDF) Under ???Lock and Key??? Control with L

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

BBa_J70604
BBa_J70604 Version 1 (Component)
J70589 cut with BsaXI and with rbs, 6his inserts (J70559-f1i,f2i,r2i,r2i) added

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

BBa_K1363200
BBa_K1363200 Version 1 (Component)
Anti-LPS factor(LALF) regulated by lacI

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.