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Showing 1201 - 1232 of 1232 result(s)
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Public
BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter
Public
BBa_K1178000
BBa_K1178000 Version 1 (Component)
tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon
Public
BBa_J70622
BBa_J70622 Version 1 (Component)
RFC12 cMyc Tag Head Domain
Public
BBa_J70624
BBa_J70624 Version 1 (Component)
RFC12 FLAG Tag Head Domain
Public
BBa_K294205
BBa_K294205 Version 1 (Component)
This is a coding sequence of heat shock protein from E.coli
Public
IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon
Public
BBa_K251000
BBa_K251000 Version 1 (Component)
coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)
Public
Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of
Public
BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock
Public
BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock
Public
BBa_K1088007
BBa_K1088007 Version 1 (Component)
E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)
Public
mccA encodes Cystathionine beta-synthase for the reverse transsulfuration pathway
module_BO_32628_encodes_BO_26539 Version 1 (Module)

Public
BBa_I13035
BBa_I13035 Version 1 (Component)
3OC<sub>6</sub>HSL Receiver Device with Inducible Control of LuxR and a YFP Output device
Public
BBa_K944001
BBa_K944001 Version 1 (Component)
Transcriptional Regulator for Cyanide Inducible Promoter
Public
BBa_K1954007
BBa_K1954007 Version 1 (Component)
Green Light Inducible bacteriocin Device (GLID)
Public
BBa_K1952012
BBa_K1952012 Version 1 (Component)
Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter
Public
BBa_K1323019
BBa_K1323019 Version 1 (Component)
Hfq expression cassette under a xylose inducible promoter
Public
BBa_K2092002
BBa_K2092002 Version 1 (Component)
PalcA, improved alcR inducible promoter from A. nidulans
Public
BBa_K590046
BBa_K590046 Version 1 (Component)
AAR-PSB3K3-Lac Inducible w/o LacI
Public
BBa_K638201
BBa_K638201 Version 1 (Component)
Arabinose inducible Poly-His Reflectin A1 generator
Public
BBa_K1088010
BBa_K1088010 Version 1 (Component)
E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib
Public
BBa_K418001
BBa_K418001 Version 1 (Component)
From partsregistry.org IPTG inducible Lac promoter cassette
Public
Prom/RBS
BBa_K262000 Version 1 (Component)
BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.
Public
BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)
Public
BBa_K1968009
BBa_K1968009 Version 1 (Component)
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
Public
AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease
Public
BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
Public
pSBBs0K
BBa_K823026 Version 1 (Component)
pSB<sub>Bs</sub>0K-P<sub>spac</sub> (replicative Bacillus subtilis expression vector; IPTG inducible
Public
BBa_K1075003
BBa_K1075003 Version 1 (Component)
Promoter(const.)-AraC-Term-pBAD-RBS34 (Arabinose inducable promoter system)
Public
BBa_K2020051
BBa_K2020051 Version 1 (Component)
wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G
Public
Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Showing 1201 - 1232 of 1232 result(s)
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