BBa_K1189030BBa_K1189030 Version 1 (Component)TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter
BBa_K1178000BBa_K1178000 Version 1 (Component)tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon
BBa_J70622BBa_J70622 Version 1 (Component)RFC12 cMyc Tag Head Domain
BBa_J70624BBa_J70624 Version 1 (Component)RFC12 FLAG Tag Head Domain
BBa_K294205BBa_K294205 Version 1 (Component)This is a coding sequence of heat shock protein from E.coli
IodoY RSBBa_K1416001 Version 1 (Component)The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon
BBa_K251000BBa_K251000 Version 1 (Component)coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)
Adapter BiBBa_K1807015 Version 1 (Component)This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of
BBa_K175035BBa_K175035 Version 1 (Component)Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock
BBa_K175034BBa_K175034 Version 1 (Component)Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock
BBa_K1088007BBa_K1088007 Version 1 (Component)E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)
BBa_I13035BBa_I13035 Version 1 (Component)3OC<sub>6</sub>HSL Receiver Device with Inducible Control of LuxR and a YFP Output device
BBa_K944001BBa_K944001 Version 1 (Component)Transcriptional Regulator for Cyanide Inducible Promoter
BBa_K1954007BBa_K1954007 Version 1 (Component)Green Light Inducible bacteriocin Device (GLID)
BBa_K1952012BBa_K1952012 Version 1 (Component)Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter
BBa_K1323019BBa_K1323019 Version 1 (Component)Hfq expression cassette under a xylose inducible promoter
BBa_K2092002BBa_K2092002 Version 1 (Component)PalcA, improved alcR inducible promoter from A. nidulans
BBa_K590046BBa_K590046 Version 1 (Component)AAR-PSB3K3-Lac Inducible w/o LacI
BBa_K638201BBa_K638201 Version 1 (Component)Arabinose inducible Poly-His Reflectin A1 generator
BBa_K1088010BBa_K1088010 Version 1 (Component)E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib
BBa_K418001BBa_K418001 Version 1 (Component)From partsregistry.org IPTG inducible Lac promoter cassette
Prom/RBSBBa_K262000 Version 1 (Component)BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.
BBa_K1088057BBa_K1088057 Version 1 (Component)T25 domain of bacterial two-hybrid system (IPTG inducible)
BBa_K1968009BBa_K1968009 Version 1 (Component)PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
AraC_TEV-FBBa_K627008 Version 1 (Component)Fusion part of arabinose-inducible induction system and the TEV protease
BBa_K563053BBa_K563053 Version 1 (Component)vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
pSBBs0KBBa_K823026 Version 1 (Component)pSB<sub>Bs</sub>0K-P<sub>spac</sub> (replicative Bacillus subtilis expression vector; IPTG inducible
BBa_K1075003BBa_K1075003 Version 1 (Component)Promoter(const.)-AraC-Term-pBAD-RBS34 (Arabinose inducable promoter system)
BBa_K2020051BBa_K2020051 Version 1 (Component)wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.