BBa_K1531004
BBa_K1531004 Version 1 (Component)
Bovine beta casein (B variant) with full alpha-factor yeast secretion signal

BBa_K222002
BBa_K222002 Version 1 (Component)
project of a Mid1 gene disrupted from 489 to 2021 nt

BBa_K1051801
BBa_K1051801 Version 1 (Component)
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

BBa_M31539
BBa_M31539 Version 1 (Component)
These are the genes in M13K07 responsible for forming the 'tip' of the phage.

BBa_M1355
BBa_M1355 Version 1 (Component)
RS1

BBa_K496004
BBa_K496004 Version 1 (Component)
Reporter of Salmonella secretion signal with NLS, GFP, etc

BBa_K563055
BBa_K563055 Version 1 (Component)
pSB1A11, reconstructed from pSB1A2 with NcoI, XhoI, BamHI and PstI

BBa_K1796113
BBa_K1796113 Version 1 (Component)
two Restriction Enzyme cutting sites(BamHI and HindIII)

BBa_S03552
BBa_S03552 Version 1 (Component)
S03145:I13507

BBa_B1110
BBa_B1110 Version 1 (Component)
B1010 I13507

BBa_B1108
BBa_B1108 Version 1 (Component)
B1008 I13507

BBa_B1102
BBa_B1102 Version 1 (Component)
B1002 I13507

BBa_S03819
BBa_S03819 Version 1 (Component)
S03818:I13507

BBa_B1105
BBa_B1105 Version 1 (Component)
B1005 I13507

BBa_B1106
BBa_B1106 Version 1 (Component)
B1006 I13507

BBa_B1107
BBa_B1107 Version 1 (Component)
B1007 I13507

BBa_B1103
BBa_B1103 Version 1 (Component)
B1003 I13507

BBa_B1101
BBa_B1101 Version 1 (Component)
B1001 I13507

BBa_B1104
BBa_B1104 Version 1 (Component)
B1004 I13507

BBa_B1109
BBa_B1109 Version 1 (Component)
B1009 I13507

BBa_K2018023
BBa_K2018023 Version 1 (Component)
hlyA secretion tag

BBa_K1758204
BBa_K1758204 Version 1 (Component)
His-tagged blc repressor fused to superfolder GFP under the control of the T7 promoter

BBa_K1761001
BBa_K1761001 Version 1 (Component)
Outer Membrane Protein X (OmpX) with BamHI-linker and mNeonGreen

BBa_J64029
BBa_J64029 Version 1 (Component)
sopD secretion signal

BBa_J22101
BBa_J22101 Version 1 (Component)
Lac Y gene

BBa_K888000
BBa_K888000 Version 1 (Component)
L2 protein of adhesion to silica

BBa_S04188
BBa_S04188 Version 1 (Component)
J23119:I13507

BBa_K189103
BBa_K189103 Version 1 (Component)
Genome of GenSniper via Top-Down approach

BBa_K1420000
BBa_K1420000 Version 1 (Component)
Mer operon, biological system found to detoxify organic and inorganic forms of mercury

BBa_M36580
BBa_M36580 Version 1 (Component)
LasR/PAI complex sensor

BBa_K215002
BBa_K215002 Version 1 (Component)
pLac+RBS+Secretion Signal and Streptavidin Binding Tags

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K640004
BBa_K640004 Version 1 (Component)
Device for conjugation of plasmids from E. coli to pseudomonas

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_J64027
BBa_J64027 Version 1 (Component)
sopB Secretion Signal

BBa_J64012
BBa_J64012 Version 1 (Component)
SipA Secretion Signal

BBa_J64016
BBa_J64016 Version 1 (Component)
sopE2 Secretion Signal

BBa_J64025
BBa_J64025 Version 1 (Component)
sigE Secretion Chaperone

BBa_J64023
BBa_J64023 Version 1 (Component)
sipC Secretion Signal

BBa_J64014
BBa_J64014 Version 1 (Component)
sopA Secretion Signal

BBa_J64017
BBa_J64017 Version 1 (Component)
invJ Secretion Signal

BBa_K957005
BBa_K957005 Version 1 (Component)
Constitutive expression of nah Operon for degradation of naphthalene to salicylate

BBa_K880002
BBa_K880002 Version 1 (Component)
GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.

BBa_K1150038
BBa_K1150038 Version 1 (Component)
uniCAS RNAimer to target VEGF3

BBa_K1150037
BBa_K1150037 Version 1 (Component)
uniCAS RNaimer to target VEGF2

Linker
BBa_K1088051 Version 1 (Component)
10 aa linker with BamHI restriction site and TEV recognition site

BBa_M31377
BBa_M31377 Version 1 (Component)
M13K07 g7 ORF -Promoter g8 -RBS g9 -ORF g9

COB termin
BBa_K809004 Version 1 (Component)
Terminator of Yeast mitochondrial gene COB

BBa_I20260
BBa_I20260 Version 1 (Component)
Measurement Kit Test of J23101

BBa_K266006
BBa_K266006 Version 1 (Component)
PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP